Recombinant Human Apolipoprotein A I is a Human Full Length protein, in the 1 to 250 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
D E P P Q S P W D R V K D L A T V Y V D V L K D S G R D Y V S Q F E G S A L G K Q L N L K L L D N W D S V T S T F S K L R E Q L G P V T Q E F W D N L E K E T E G L R Q E M S K D L E E V K A K V Q P Y L D D F Q K K W Q E E M E L Y R Q K V E P L R A E L Q E G A R Q K L H E L Q E K L S P L G E E M R D R A R A H V D A L R T H L A P Y S D E L R Q R L A A R L E A L K E N G G A R L A E Y H A K A T E H L S T L S E K A K P A L E D L R Q G L L P V L E S F K V S F L S A L E E Y T K K L N T Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Alternative names
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1
Recommended products
Recombinant Human Apolipoprotein A I is a Human Full Length protein, in the 1 to 250 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P02647-1
- Animal free
- No
- Species
- Human
- Reconstitution
- It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- D E P P Q S P W D R V K D L A T V Y V D V L K D S G R D Y V S Q F E G S A L G K Q L N L K L L D N W D S V T S T F S K L R E Q L G P V T Q E F W D N L E K E T E G L R Q E M S K D L E E V K A K V Q P Y L D D F Q K K W Q E E M E L Y R Q K V E P L R A E L Q E G A R Q K L H E L Q E K L S P L G E E M R D R A R A H V D A L R T H L A P Y S D E L R Q R L A A R L E A L K E N G G A R L A E Y H A K A T E H L S T L S E K A K P A L E D L R Q G L L P V L E S F K V S F L S A L E E Y T K K L N T Q
- Accession
- P02647
- Protein length
- Full Length
- Predicted molecular weight
- 29 kDa
- Amino acids
- 1 to 250
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
ab155731 was lyophilized from 0.22 µm filtered solution.
General info
- Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
- Sequence similarities
Belongs to the apolipoprotein A1/A4/E family.
- Post-translational modifications
Glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
- Biological function summary
ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.
- Pathways
The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.
- Associated diseases and disorders
Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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SDS-PAGE - Recombinant Human Apolipoprotein A I (ab155731)
SDS-PAGE analysis of reduced ab155731 stained overnight with Coomassie Blue.
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