Recombinant Human Apolipoprotein A V/APOA5 is a Human Full Length protein, in the 24 to 366 aa range, expressed in HEK 293, with >70% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Purity
- >70% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
R K G F W D Y F S Q T S G D K G R V E Q I H Q Q K M A R E P A T L K D S L E Q D L N N M N K F L E K L R P L S G S E A P R L P Q D P V G M R R Q L Q E E L E E V K A R L Q P Y M A E A H E L V G W N L E G L R Q Q L K P Y T M D L M E Q V A L R V Q E L Q E Q L R V V G E D T K A Q L L G G V D E A W A L L Q G L Q S R V V H H T G R F K E L F H P Y A E S L V S G I G R H V Q E L H R S V A P H A P A S P A R L S R C V Q V L S R K L T L K A K A L H A R I Q Q N L D Q L R E E L S R A F A G T G T E E G A G P D P Q M L S E E V R Q R L Q A F R Q D T Y L Q I A A F T R A I D Q E T E E V Q Q Q L A P P P P G H S A F A P E F Q Q T D S G K V L S K L Q A R L D D L W E D I T H S L H D Q G H S H L G D P A A A D Y K D D D D K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed:17326667).
Alternative names
RAP3, UNQ411/PRO773, APOA5, Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3
Recommended products
Recombinant Human Apolipoprotein A V/APOA5 is a Human Full Length protein, in the 24 to 366 aa range, expressed in HEK 293, with >70% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
- >70% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q6Q788-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in 200 µL of water
- Concentration
- Storage buffer
Constituents: 0.29% Sodium chloride, 0.24% Tris
Sequence info
Amino acid sequence
- R K G F W D Y F S Q T S G D K G R V E Q I H Q Q K M A R E P A T L K D S L E Q D L N N M N K F L E K L R P L S G S E A P R L P Q D P V G M R R Q L Q E E L E E V K A R L Q P Y M A E A H E L V G W N L E G L R Q Q L K P Y T M D L M E Q V A L R V Q E L Q E Q L R V V G E D T K A Q L L G G V D E A W A L L Q G L Q S R V V H H T G R F K E L F H P Y A E S L V S G I G R H V Q E L H R S V A P H A P A S P A R L S R C V Q V L S R K L T L K A K A L H A R I Q Q N L D Q L R E E L S R A F A G T G T E E G A G P D P Q M L S E E V R Q R L Q A F R Q D T Y L Q I A A F T R A I D Q E T E E V Q Q Q L A P P P P G H S A F A P E F Q Q T D S G K V L S K L Q A R L D D L W E D I T H S L H D Q G H S H L G D P A A A D Y K D D D D K
- Accession
- Q6Q788
- Protein length
- Full Length
- Predicted molecular weight
- 40.1 kDa
- Amino acids
- 24 to 366
- Nature
- Recombinant
- Tags
- DDDDK tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purity determined by densitometric image analysis
General info
- Function
Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed:17326667).
- Sequence similarities
Belongs to the apolipoprotein A1/A4/E family.
- Post-translational modifications
Phosphorylated by FAM20C in the extracellular medium.
- Subcellular localisation
- Early endosome, Late endosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Apolipoprotein A V
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein A V also known as APOA5 plays a critical role in lipid metabolism. This protein possesses a molecular weight of approximately 39 kDa and is mainly expressed in the liver. APOA5 modulates the plasma triglyceride levels by enhancing the uptake and clearance of triglyceride-rich lipoproteins. The protein is located at the surface of these lipoproteins facilitating their interaction with receptors and enzymes like lipoprotein lipase.
- Biological function summary
APOA5 influences triglyceride metabolism and is part of the HDL particle complex. It interacts directly with lipoprotein lipase to promote the hydrolysis of triglycerides into free fatty acids. This hydrolytic action aids in reducing triglyceride levels in the plasma. APOA5 also indirectly influences very-low-density lipoprotein (VLDL) metabolism reinforcing its role in lipid regulation.
- Pathways
APOA5 integrates into the lipoprotein metabolic pathway and the PPAR signaling pathway. In the lipoprotein metabolic pathway APOA5 works with proteins like APOC3 to influence triglyceride homeostasis. This protein is modulated by PPAR-alpha helping control lipid metabolism and energy homeostasis. Its role in these pathways highlights its impact on triglyceride-rich lipoprotein metabolism positioning it as an important player in maintaining lipid balance.
- Associated diseases and disorders
APOA5 strongly associates with hypertriglyceridemia and cardiovascular disease. Variations in the APOA5 gene can lead to abnormal plasma triglyceride levels contributing to the development of hypertriglyceridemia. Additionally APOA5's connection with APOE proteins through lipid metabolism pathways can influence susceptibility to cardiovascular disorders. Understanding the interactions and mutations within APOA5 provides insights into the mechanisms contributing to these disease states.
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1 product image
SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 (ab113861)
12% SDS-PAGE separation of Human Apolipoprotein A V/APOA5
1.reduced and boiled sample, 5μg/lane
2.non-reduced and non-boiled sample, 5μg/lane
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