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AB113861

Recombinant Human Apolipoprotein A V/APOA5

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(1 Publication)

Recombinant Human Apolipoprotein A V/APOA5 is a Human Full Length protein, in the 24 to 366 aa range, expressed in HEK 293 cells, with >70%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

RAP3, UNQ411/PRO773, APOA5, Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3

1 Images
SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 (AB113861)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 (AB113861)

12% SDS-PAGE separation of Human Apolipoprotein A V/APOA5
1.reduced and boiled sample, 5μg/lane
2.non-reduced and non-boiled sample, 5μg/lane

Key facts

Purity

>70% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

DDDDK tag C-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

Q6Q788

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 200 µL of water

Storage buffer

Constituents: 0.29% Sodium chloride, 0.24% Tris

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Apolipoprotein A V
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Sequence info

[{"sequence":"RKGFWDYFSQTSGDKGRVEQIHQQKMAREPATLKDSLEQDLNNMNKFLEKLRPLSGSEAPRLPQDPVGMRRQLQEELEEVKARLQPYMAEAHELVGWNLEGLRQQLKPYTMDLMEQVALRVQELQEQLRVVGEDTKAQLLGGVDEAWALLQGLQSRVVHHTGRFKELFHPYAESLVSGIGRHVQELHRSVAPHAPASPARLSRCVQVLSRKLTLKAKALHARIQQNLDQLREELSRAFAGTGTEEGAGPDPQMLSEEVRQRLQAFRQDTYLQIAAFTRAIDQETEEVQQQLAPPPPGHSAFAPEFQQTDSGKVLSKLQARLDDLWEDITHSLHDQGHSHLGDPAAADYKDDDDK","proteinLength":"Full Length","predictedMolecularWeight":"40.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":366,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q6Q788","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A V also known as APOA5 plays a critical role in lipid metabolism. This protein possesses a molecular weight of approximately 39 kDa and is mainly expressed in the liver. APOA5 modulates the plasma triglyceride levels by enhancing the uptake and clearance of triglyceride-rich lipoproteins. The protein is located at the surface of these lipoproteins facilitating their interaction with receptors and enzymes like lipoprotein lipase.
Biological function summary

APOA5 influences triglyceride metabolism and is part of the HDL particle complex. It interacts directly with lipoprotein lipase to promote the hydrolysis of triglycerides into free fatty acids. This hydrolytic action aids in reducing triglyceride levels in the plasma. APOA5 also indirectly influences very-low-density lipoprotein (VLDL) metabolism reinforcing its role in lipid regulation.

Pathways

APOA5 integrates into the lipoprotein metabolic pathway and the PPAR signaling pathway. In the lipoprotein metabolic pathway APOA5 works with proteins like APOC3 to influence triglyceride homeostasis. This protein is modulated by PPAR-alpha helping control lipid metabolism and energy homeostasis. Its role in these pathways highlights its impact on triglyceride-rich lipoprotein metabolism positioning it as an important player in maintaining lipid balance.

APOA5 strongly associates with hypertriglyceridemia and cardiovascular disease. Variations in the APOA5 gene can lead to abnormal plasma triglyceride levels contributing to the development of hypertriglyceridemia. Additionally APOA5's connection with APOE proteins through lipid metabolism pathways can influence susceptibility to cardiovascular disorders. Understanding the interactions and mutations within APOA5 provides insights into the mechanisms contributing to these disease states.
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Specifications

Form

Lyophilized

Additional notes

Purity determined by densitometric image analysis

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General info

Function

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).

Sequence similarities

Belongs to the apolipoprotein A1/A4/E family.

Post-translational modifications

Phosphorylated by FAM20C in the extracellular medium.

Subcellular localisation

Early endosome

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Product protocols

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Target data

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).
See full target information APOA5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity 56:2523-2541.e8 PubMed37924812

2023

Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Miao,Cong Jiang,Winston Y Chang,Haiwei Zhang,Jinsu An,Felicia Ho,Pengcheng Chen,Han Zhang,Caroline Junqueira,Dulguun Amgalan,Felix G Liang,Junbing Zhang,Charles L Evavold,Iva Hafner-Bratkovič,Zhibin Zhang,Pietro Fontana,Shiyu Xia,Markus Waldeck-Weiermair,Youdong Pan,Thomas Michel,Liron Bar-Peled,Hao Wu,Jonathan C Kagan,Richard N Kitsis,Peng Zhang,Xing Liu,Judy Lieberman
View all publications
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Complementary Products

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