Recombinant Human APPBP1 protein
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Recombinant Human APPBP1 protein is a Human Full Length protein, in the 1 to 534 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
View Alternative Names
APPBP1, HPP1, NAE1, NEDD8-activating enzyme E1 regulatory subunit, Amyloid protein-binding protein 1, Proto-oncogene protein 1, APP-BP1
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human APPBP1 protein (AB116199)
15% SDS-PAGE analysis of 3 µg ab116199.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
APPBP1 interacts within a complex known as the NEDD8-activating enzyme. This complex is essential in the neddylation pathway influencing protein degradation through the ubiquitin-proteasome system. By conjugating NEDD8 to target proteins APPBP1 controls many processes such as cell cycle regulation signal transduction and transcription. It is of special interest in research involving cellular signaling because neddylation modifies the activity and stability of many substrate proteins involved in these critical cellular functions.
Pathways
The involvement of APPBP1 in ubiquitin-like protein pathways highlights its influence on cellular homeostasis. The protein is associated with the neddylation and ubiquitination pathways influencing other members such as cullin proteins involved in cell cycle control and other cellular processes. APPBP1-related interactions play a significant role in ensuring the timely degradation of specific proteins maintaining balance within the cell's regulatory mechanisms.
Specifications
Form
Liquid
Additional notes
ab116199 was purified using conventional chromatography techniques.
General info
Function
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development.
Sequence similarities
Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.
Post-translational modifications
Ubiquitinated by TRIP12, leading to its degradation by the proteasome.
Target data
Complementary Products
Product promise
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