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AB93630

Recombinant Human Aprataxin protein (His tag N-Terminus)

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Recombinant Human Aprataxin protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 168 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

AXA1, APTX, Aprataxin, Forkhead-associated domain histidine triad-like protein, FHA-HIT

1 Images
SDS-PAGE - Recombinant Human Aprataxin protein (AB93630)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Aprataxin protein (AB93630)

15% SDS-PAGE showing ab93630 at approximately 23.9kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q7Z2E3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWAGSMQDPKMQVYKDEQVVVIKDKYPKARYHWLVLPWTSISSLKAVAREHLELLKHMHTVGEKVIVDFAGSSKLRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNKKHWNSFNTEYFLESQAVIEMVQEAGRVTVRDGMPELLKLPLRCHECQQLLPSIPQLKEHLRKHWTQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":168,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q7Z2E3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aprataxin also known as APTX is a protein involved in DNA repair. It belongs to the histidine triad superfamily and has a molecular mass of approximately 40 kDa. The protein is expressed in various tissues including the brain liver and kidney. Aprataxin's main role involves the repair of DNA strand breaks by removing abnormal DNA termini. It restores DNA integrity which is critical for maintaining cellular functions.
Biological function summary

Aprataxin plays an important role in the DNA single-strand break repair processes. It functions within the DNA repair machinery often as part of complexes with other repair proteins. This protein mainly interacts with other partners like XRCC1 and PARP1 to facilitate accurate DNA repair. Its interaction ensures proper response to DNA damage safeguarding the genome's stability and function.

Pathways

Aprataxin is integrally connected to the base excision repair (BER) pathway and the DNA damage response pathway. These pathways are fundamental mechanisms for repairing damaged DNA and preserving cell viability. Within these pathways Aprataxin closely collaborates with related proteins like XRCC1 and PARP1. These associations highlight Aprataxin's role in ensuring the efficiency and success of DNA repair processes.

Mutations or malfunctions in Aprataxin are linked to neurological conditions such as ataxia with oculomotor apraxia 1 (AOA1) and spinocerebellar ataxia with axonal neuropathy (SCAN1). These disorders are marked by progressive loss of movement coordination and peripheral neuropathy. In the context of these diseases Aprataxin interacts with proteins involved in neuronal maintenance and protection such as TDP-43 and p53. Disruptions in such interactions often result in the observed pathological manifestations.
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Specifications

Form

Liquid

Additional notes

ab93630 is purified using conventional chromatography techniques.

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General info

Function

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair (PubMed : 15044383, PubMed : 15380105, PubMed : 16964241, PubMed : 17276982, PubMed : 24362567). Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species (PubMed : 16964241, PubMed : 24362567). Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed : 16964241, PubMed : 17276982, PubMed : 24362567). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (PubMed : 16547001). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).

Subcellular localisation

Nucleus

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Product protocols

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Target data

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair (PubMed : 15044383, PubMed : 15380105, PubMed : 16964241, PubMed : 17276982, PubMed : 24362567). Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species (PubMed : 16964241, PubMed : 24362567). Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed : 16964241, PubMed : 17276982, PubMed : 24362567). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (PubMed : 16547001). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).
See full target information APTX
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Complementary Products

Primary Antibodies

AB154413

Anti-Aprataxin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Aprataxin antibody (AB154413)

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Cell Lines & Lysates

AB265118

Human APTX (Aprataxin) knockout HeLa cell line

Western blot - Human APTX (Aprataxin) knockout HeLa cell line (AB265118)

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Cell Lines & Lysates

AB257837

Human APTX (Aprataxin) knockout HeLa cell lysate

Western blot - Human APTX (Aprataxin) knockout HeLa cell lysate (AB257837)

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Proteins & Peptides

AB191960

Recombinant Human B4GALT3 protein

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