Recombinant Human Aprataxin protein is a Human Full Length protein, in the 1 to 168 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W A G S M Q D P K M Q V Y K D E Q V V V I K D K Y P K A R Y H W L V L P W T S I S S L K A V A R E H L E L L K H M H T V G E K V I V D F A G S S K L R F R L G Y H A I P S M S H V H L H V I S Q D F D S P C L K N K K H W N S F N T E Y F L E S Q A V I E M V Q E A G R V T V R D G M P E L L K L P L R C H E C Q Q L L P S I P Q L K E H L R K H W T Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair (PubMed:15044383, PubMed:15380105, PubMed:16964241, PubMed:17276982, PubMed:24362567). Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species (PubMed:16964241, PubMed:24362567). Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed:16964241, PubMed:17276982, PubMed:24362567). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (PubMed:16547001). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).
Alternative names
AXA1, APTX, Aprataxin, Forkhead-associated domain histidine triad-like protein, FHA-HIT
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Recombinant Human Aprataxin protein is a Human Full Length protein, in the 1 to 168 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q7Z2E3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.00174% PMSF
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W A G S M Q D P K M Q V Y K D E Q V V V I K D K Y P K A R Y H W L V L P W T S I S S L K A V A R E H L E L L K H M H T V G E K V I V D F A G S S K L R F R L G Y H A I P S M S H V H L H V I S Q D F D S P C L K N K K H W N S F N T E Y F L E S Q A V I E M V Q E A G R V T V R D G M P E L L K L P L R C H E C Q Q L L P S I P Q L K E H L R K H W T Q
- Accession
- Q7Z2E3
- Protein length
- Full Length
- Amino acids
- 1 to 168
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab93630 is purified using conventional chromatography techniques.
General info
- Function
DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair (PubMed:15044383, PubMed:15380105, PubMed:16964241, PubMed:17276982, PubMed:24362567). Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species (PubMed:16964241, PubMed:24362567). Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed:16964241, PubMed:17276982, PubMed:24362567). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (PubMed:16547001). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).
- Subcellular localisation
- Nucleus, Nucleoplasm, Nucleolus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aprataxin also known as APTX is a protein involved in DNA repair. It belongs to the histidine triad superfamily and has a molecular mass of approximately 40 kDa. The protein is expressed in various tissues including the brain liver and kidney. Aprataxin's main role involves the repair of DNA strand breaks by removing abnormal DNA termini. It restores DNA integrity which is critical for maintaining cellular functions.
- Biological function summary
Aprataxin plays an important role in the DNA single-strand break repair processes. It functions within the DNA repair machinery often as part of complexes with other repair proteins. This protein mainly interacts with other partners like XRCC1 and PARP1 to facilitate accurate DNA repair. Its interaction ensures proper response to DNA damage safeguarding the genome's stability and function.
- Pathways
Aprataxin is integrally connected to the base excision repair (BER) pathway and the DNA damage response pathway. These pathways are fundamental mechanisms for repairing damaged DNA and preserving cell viability. Within these pathways Aprataxin closely collaborates with related proteins like XRCC1 and PARP1. These associations highlight Aprataxin's role in ensuring the efficiency and success of DNA repair processes.
- Associated diseases and disorders
Mutations or malfunctions in Aprataxin are linked to neurological conditions such as ataxia with oculomotor apraxia 1 (AOA1) and spinocerebellar ataxia with axonal neuropathy (SCAN1). These disorders are marked by progressive loss of movement coordination and peripheral neuropathy. In the context of these diseases Aprataxin interacts with proteins involved in neuronal maintenance and protection such as TDP-43 and p53. Disruptions in such interactions often result in the observed pathological manifestations.
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1 product image
SDS-PAGE - Recombinant Human Aprataxin protein (ab93630)
15% SDS-PAGE showing ab93630 at approximately 23.9kDa (3μg).
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