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AB105129

Recombinant Human APT-2 protein

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Recombinant Human APT-2 protein is a Human Full Length protein, in the 1 to 231 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

APT2, LYPLA2, Acyl-protein thioesterase 2, APT-2, Lysophospholipase II, Palmitoyl-protein hydrolase, LPL-II, LysoPLA II

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SDS-PAGE - Recombinant Human APT-2 protein (AB105129)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human APT-2 protein (AB105129)

15% SDS-PAGE showing ab105129 at approximately 26.9kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O95372

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein previously labeled as LYPLA2.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMCGNTMSVPLLTDAATVSGAERETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTCPHPLAGIVALSCWLPLHRAFPQAANGSAKDLAILQCHGELDPMVPVRFGALTAEKLRSVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLLPPV","proteinLength":"Full Length","predictedMolecularWeight":"26.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":231,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95372","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APT-2 also known as lysophospholipase A2 (lypla2) is an enzyme involved in lipid metabolism. APT-2 has a molecular mass of approximately 25 kDa and is expressed in various tissues including the brain heart and muscle. As an important member of the lipid acyl hydrolase family it removes fatty acids from lysophospholipids. Researchers often use the names anti-APT and anti-ApT in antibody studies targeting this enzyme. APT-2 identification in these tissues highlights its importance in lipid processing and suggests its broader functional implications.
Biological function summary

APT-2 plays a critical role in maintaining phospholipid homeostasis contributing to membrane fluidity and signal transduction. It is involved in hydrolyzing lysophospholipids into lysoglycerophospholipids and free fatty acids. Not part of a complex APT-2 acts as an individual enzyme handling specific substrates in this context. Its activity regulates levels of lipid signaling molecules influencing cellular responses to environmental cues. APT-2 also contributes to energy storage via metabolism of lipid substrates linking its enzymatic activity to broader metabolic pathways.

Pathways

APT-2 holds significance in both metabolic and signaling pathways specifically the glycerophospholipid metabolism and lysophospholipid signaling pathways. It catalyzes reactions key to these pathways and intersects with proteins like phospholipase A2 and PLA2G6. Glycerophospholipid metabolism sees APT-2 breaking down molecules to sustain membrane integrity and cellular communication processes. In lysophospholipid signaling its activity regulates bioactive lipid levels which impact a variety of cellular signaling events showing a well-integrated role in cellular physiology.

The dysfunction of APT-2 links to neurodegenerative diseases and cardiovascular disorders. Altered APT-2 activity is associated with Alzheimer's disease due to disrupted lipid homeostasis and membrane composition. The enzyme's interaction with proteins like phospholipase A2 highlights its involvement in these neurodegenerative processes. Furthermore cardiovascular health implications arise from its role in lysophospholipid metabolism and associated inflammatory responses. Aberrations in APT-2 function can modulate pathways that exacerbate atherosclerosis further connecting its activity to disease states.
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Specifications

Form

Liquid

Additional notes

ab105129 is purified using conventional chromatography techniques.

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General info

Function

Acts as an acyl-protein thioesterase hydrolyzing fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins, GSDMD, GAP43, ZDHHC6 or HRAS (PubMed : 21152083, PubMed : 28826475). Deacylates GAP43 (PubMed : 21152083). Mediates depalmitoylation of ZDHHC6 (PubMed : 28826475). Has lysophospholipase activity (PubMed : 25301951). Hydrolyzes prostaglandin glycerol esters (PG-Gs) in the following order prostaglandin D2-glycerol ester (PGD2-G) > prostaglandin E2 glycerol ester (PGE2-G) > prostaglandin F2-alpha-glycerol ester (PGF2-alpha-G) (PubMed : 25301951). Hydrolyzes 1-arachidonoylglycerol but not 2-arachidonoylglycerol or arachidonoylethanolamide (PubMed : 25301951).

Sequence similarities

Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.

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Product protocols

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Target data

Acts as an acyl-protein thioesterase hydrolyzing fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins, GSDMD, GAP43, ZDHHC6 or HRAS (PubMed : 21152083, PubMed : 28826475). Deacylates GAP43 (PubMed : 21152083). Mediates depalmitoylation of ZDHHC6 (PubMed : 28826475). Has lysophospholipase activity (PubMed : 25301951). Hydrolyzes prostaglandin glycerol esters (PG-Gs) in the following order prostaglandin D2-glycerol ester (PGD2-G) > prostaglandin E2 glycerol ester (PGE2-G) > prostaglandin F2-alpha-glycerol ester (PGF2-alpha-G) (PubMed : 25301951). Hydrolyzes 1-arachidonoylglycerol but not 2-arachidonoylglycerol or arachidonoylethanolamide (PubMed : 25301951).
See full target information LYPLA2
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Complementary Products

Primary Antibodies

AB151578

Anti-APT-2 antibody

Western blot - Anti-APT-2 antibody (AB151578)

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Cell Lines & Lysates

AB266696

Human LYPLA2 (APT-2) knockout HEK-293T cell line

Sanger Sequencing - Human LYPLA2 (APT-2) knockout HEK-293T cell line (AB266696)

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Cell Lines & Lysates

AB258950

Human LYPLA2 (APT-2) knockout HEK-293T cell lysate

Sanger Sequencing - Human LYPLA2 (APT-2) knockout HEK-293T cell lysate (AB258950)

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