Recombinant Human Arc protein is a Human Full Length protein, in the 1 to 396 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M E L D H R T S G G L H A Y P G P R G G Q V A K P N V I L Q I G K C R A E M L E H V R R T H R H L L A E V S K Q V E R E L K G L H R S V G K L E S N L D G Y V P T S D S Q R W K K S I K A C L C R C Q E T I A N L E R W V K R E M H V W R E V F Y R L E R W A D R L E S T G G K Y P V G S E S A R H T V S V G V G G P E S Y C H E A D G Y D Y T V S P Y A I T P P P A A G E L P G Q E P A E A Q Q Y Q P W V P G E D G Q P S P G V D T Q I F E D P R E F L S H L E E Y L R Q V G G S E E Y W L S Q I Q N H M N G P A K K W W E F K Q G S V K N W V E F K K E F L Q Y S E G T L S R E A I Q R E L D L P Q K Q G E P L D Q F L W R K R D L Y Q T L Y V D A D E E E I I Q Y V V G T L Q P K L K R F L R H P L P K T L E Q L I Q R G M E V Q D D L E Q A A E P A G P H L P V E D E A E T L T P A P N S E S V A S D R T Q P E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (By similarity). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (By similarity). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (By similarity).
Alternative names
KIAA0278, ARC, Activity-regulated cytoskeleton-associated protein, hArc, Activity-regulated gene 3.1 protein homolog, ARC/ARG3.1, Arg3.1
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Recombinant Human Arc protein is a Human Full Length protein, in the 1 to 396 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q7LC44-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M E L D H R T S G G L H A Y P G P R G G Q V A K P N V I L Q I G K C R A E M L E H V R R T H R H L L A E V S K Q V E R E L K G L H R S V G K L E S N L D G Y V P T S D S Q R W K K S I K A C L C R C Q E T I A N L E R W V K R E M H V W R E V F Y R L E R W A D R L E S T G G K Y P V G S E S A R H T V S V G V G G P E S Y C H E A D G Y D Y T V S P Y A I T P P P A A G E L P G Q E P A E A Q Q Y Q P W V P G E D G Q P S P G V D T Q I F E D P R E F L S H L E E Y L R Q V G G S E E Y W L S Q I Q N H M N G P A K K W W E F K Q G S V K N W V E F K K E F L Q Y S E G T L S R E A I Q R E L D L P Q K Q G E P L D Q F L W R K R D L Y Q T L Y V D A D E E E I I Q Y V V G T L Q P K L K R F L R H P L P K T L E Q L I Q R G M E V Q D D L E Q A A E P A G P H L P V E D E A E T L T P A P N S E S V A S D R T Q P E
- Accession
- Q7LC44
- Protein length
- Full Length
- Predicted molecular weight
- 47.4 kDa
- Amino acids
- 1 to 396
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab140728 was purified using conventional chromatography techniques.
General info
- Function
Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (By similarity). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (By similarity). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (By similarity).
- Sequence similarities
Belongs to the ARC/ARG3.1 family.
- Post-translational modifications
Palmitoylation anchors the protein into the membrane by allowing direct insertion into the hydrophobic core of the lipid bilayer.
- Subcellular localisation
- Early endosome membrane, Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Arc protein also known as Activity-regulated cytoskeleton-associated protein weighs approximately 45 kDa. This protein expresses mainly in the brain specifically in the neurons. It is particularly active in the synaptic regions where it plays a role in synaptic plasticity. The Arc protein belongs to an emerging class of proteins that show similarities with viral proteins. Its structural and functional properties help facilitate the dynamic regulation of synapses and neuronal activity.
- Biological function summary
The Arc protein influences synaptic plasticity and memory formation. It does not operate alone; instead it interacts within complexes that regulate synaptic strength. These complexes contain other proteins critical for actin cytoskeleton remodeling which impacts synaptic efficacy. Through these interactions Arc supports the internalization of AMPA receptors playing a role in long-term depression (LTD) and long-term potentiation (LTP) of synapses essential mechanisms for learning and memory.
- Pathways
The Arc protein forms integral parts of the synaptic signaling pathways. It connects with the BDNF-TrkB signaling pathway which is important for neuronal development and synaptic modulation. Arc also relates to the mTOR signaling pathway which governs cellular growth and protein synthesis. Through these pathways Arc interacts with proteins like Neuro2a and SynGAP highlighting its comprehensive role in synaptic regulation and plasticity.
- Associated diseases and disorders
Arc protein has a connection to neurological disorders such as Alzheimer's disease and schizophrenia. Researchers found altered Arc expression and function in the brains of affected individuals. These disorders show a link to disrupted synaptic plasticity where Arc plays a significant role. Arc connects to other proteins like Neuro-2a and Caspase-3 in the pathology of these conditions indicating potential targets for therapeutic intervention.
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SDS-PAGE - Recombinant Human Arc protein (ab140728)
15% SDS-PAGE analysis of ab140728 (3μg)..
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