Recombinant Human Arfaptin-1 protein is a Human Full Length protein, in the 1 to 341 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A Q E S P K N S A A E I P V T S N G E V D D S R E H S F N R D L K H S L P S G L G L S E T Q I T S H G F D N T K E G V I E A G A F Q G G Q R T Q T K S G P V I L A D E I K N P A M E K L E L V R K W S L N T Y K C T R Q I I S E K L G R G S R T V D L E L E A Q I D I L R D N K K K Y E N I L K L A Q T L S T Q L F Q M V H T Q R Q L G D A F A D L S L K S L E L H E E F G Y N A D T Q K L L A K N G E T L L G A I N F F I A S V N T L V N K T I E D T L M T V K Q Y E S A R I E Y D A Y R T D L E E L N L G P R D A N T L P K I E Q S Q H L F Q A H K E K Y D K M R N D V S V K L K F L E E N K V K V L H N Q L V L F H N A I A A Y F A G N Q K Q L E Q T L K Q F H I K L K T P G V D A P S W L E E Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed:22981988). Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed:22981988, PubMed:9038142). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed:22981988). In turn, ARFs induce fission (PubMed:22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed:22981988).
Alternative names
Arfaptin-1, ADP-ribosylation factor-interacting protein 1, ARFIP1
Recommended products
Recombinant Human Arfaptin-1 protein is a Human Full Length protein, in the 1 to 341 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P53367-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A Q E S P K N S A A E I P V T S N G E V D D S R E H S F N R D L K H S L P S G L G L S E T Q I T S H G F D N T K E G V I E A G A F Q G G Q R T Q T K S G P V I L A D E I K N P A M E K L E L V R K W S L N T Y K C T R Q I I S E K L G R G S R T V D L E L E A Q I D I L R D N K K K Y E N I L K L A Q T L S T Q L F Q M V H T Q R Q L G D A F A D L S L K S L E L H E E F G Y N A D T Q K L L A K N G E T L L G A I N F F I A S V N T L V N K T I E D T L M T V K Q Y E S A R I E Y D A Y R T D L E E L N L G P R D A N T L P K I E Q S Q H L F Q A H K E K Y D K M R N D V S V K L K F L E E N K V K V L H N Q L V L F H N A I A A Y F A G N Q K Q L E Q T L K Q F H I K L K T P G V D A P S W L E E Q
- Accession
- P53367
- Protein length
- Full Length
- Predicted molecular weight
- 41 kDa
- Amino acids
- 1 to 341
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab187475 was purified using conventional chromatography techniques.
General info
- Function
Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed:22981988). Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed:22981988, PubMed:9038142). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed:22981988). In turn, ARFs induce fission (PubMed:22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed:22981988).
- Post-translational modifications
Phosphorylated by PRKD1; phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Arfaptin-1 also known as ARFIP1 acts mechanically by modulating membrane dynamics and cell signaling. This protein with a mass of approximately 36 kDa associates with membranes through a BAR domain which induces curvature. It interacts with small GTPases including ARF and Rac1 to facilitate various intracellular trafficking processes. Arfaptin-1 expression occurs in multiple tissue types suggesting its wide role in cellular functions.
- Biological function summary
Arfaptin-1 plays a part in intracellular transport and membrane receptor regulation. It is also recognized as a component of larger protein complexes contributing to the reorganization of actin cytoskeleton. Through its interactions with GTPases Arfaptin-1 influences endocytosis and exocytosis affecting cell surface receptor turnover. This adaptability allows it to play important roles in cellular communication and response to environmental stimuli.
- Pathways
Arfaptin-1 is integral in cellular trafficking and signal transduction pathways. It is involved in the ARF small GTPase pathway influencing vesicle formation and trafficking. Arfaptin-1 also interacts with other proteins like Rac1 in the regulation of actin dynamics impacting the actin cytoskeleton remodeling pathway. These interactions highlight its central position in coordinating cellular architecture and material transport.
- Associated diseases and disorders
Arfaptin-1 involvement links to conditions like cancer and neurological disorders. Altered expression of Arfaptin-1 has been observed in cancer cells where it affects cell migration and invasion linking with other proteins such as Rac1. In neurological disorders abnormal Arfaptin-1 function disrupts synaptic vesicle trafficking potentially interacting with proteins involved in neurodegenerative diseases. These associations emphasize its potential as a target in therapeutic strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Arfaptin-1 protein (ab187475)
15% SDS-PAGE analysis of 3 μg ab187475.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com