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AB187475

Recombinant Human Arfaptin-1 protein

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Recombinant Human Arfaptin-1 protein is a Human Full Length protein, in the 1 to 341 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Arfaptin-1, ADP-ribosylation factor-interacting protein 1, ARFIP1

1 Images
SDS-PAGE - Recombinant Human Arfaptin-1 protein (AB187475)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Arfaptin-1 protein (AB187475)

15% SDS-PAGE analysis of 3 μg ab187475.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P53367

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLKTPGVDAPSWLEEQ","proteinLength":"Full Length","predictedMolecularWeight":"41 kDa","actualMolecularWeight":null,"aminoAcidEnd":341,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P53367","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arfaptin-1 also known as ARFIP1 acts mechanically by modulating membrane dynamics and cell signaling. This protein with a mass of approximately 36 kDa associates with membranes through a BAR domain which induces curvature. It interacts with small GTPases including ARF and Rac1 to facilitate various intracellular trafficking processes. Arfaptin-1 expression occurs in multiple tissue types suggesting its wide role in cellular functions.
Biological function summary

Arfaptin-1 plays a part in intracellular transport and membrane receptor regulation. It is also recognized as a component of larger protein complexes contributing to the reorganization of actin cytoskeleton. Through its interactions with GTPases Arfaptin-1 influences endocytosis and exocytosis affecting cell surface receptor turnover. This adaptability allows it to play important roles in cellular communication and response to environmental stimuli.

Pathways

Arfaptin-1 is integral in cellular trafficking and signal transduction pathways. It is involved in the ARF small GTPase pathway influencing vesicle formation and trafficking. Arfaptin-1 also interacts with other proteins like Rac1 in the regulation of actin dynamics impacting the actin cytoskeleton remodeling pathway. These interactions highlight its central position in coordinating cellular architecture and material transport.

Arfaptin-1 involvement links to conditions like cancer and neurological disorders. Altered expression of Arfaptin-1 has been observed in cancer cells where it affects cell migration and invasion linking with other proteins such as Rac1. In neurological disorders abnormal Arfaptin-1 function disrupts synaptic vesicle trafficking potentially interacting with proteins involved in neurodegenerative diseases. These associations emphasize its potential as a target in therapeutic strategies.
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Specifications

Form

Liquid

Additional notes

ab187475 was purified using conventional chromatography techniques.

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General info

Function

Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed : 22981988). Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed : 22981988, PubMed : 9038142). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed : 22981988). In turn, ARFs induce fission (PubMed : 22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed : 22981988).

Post-translational modifications

Phosphorylated by PRKD1; phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery.

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Product protocols

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Target data

Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed : 22981988). Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed : 22981988, PubMed : 9038142). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed : 22981988). In turn, ARFs induce fission (PubMed : 22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed : 22981988).
See full target information ARFIP1
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Cell Lines & Lysates

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Cell Lines & Lysates

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Primary Antibodies

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