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AB97349

Recombinant Human Arg2 protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human Arg2 protein (His tag N-Terminus) is a Human Full Length protein, in the 23 to 354 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Arginase II, Kidney-type arginase, Non-hepatic arginase, Type II arginase, ARG2

1 Images
SDS-PAGE - Recombinant Human Arg2 protein (His tag N-Terminus) (AB97349)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Arg2 protein (His tag N-Terminus) (AB97349)

15% SDS-PAGE showing ab97349 at approximately 38.3kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P78540

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMVHSVAVIGAPFSQGQKRKGVEHGPAAIREAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISASIVYIGLRDVDPPEHFILKNYDIQYFSMRDIDRLGIQKVMERTFDLLIGKRQRPIHLSFDIDAFDPTLAPATFTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI","proteinLength":"Full Length","predictedMolecularWeight":"38.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":354,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P78540","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arginase 2 (Arg2) also known as AII is an enzyme with a molecular weight of approximately 39 kDa. It is part of the urea cycle and functions to hydrolyze L-arginine into L-ornithine and urea. Arg2 is a mitochondrial enzyme mainly expressed in the liver kidney and prostate. Its expression can extend to other tissues such as the small intestine in some conditions and its activity is important in regulating arginine availability for various physiological processes.
Biological function summary

Arg2 plays a role in nitrogen disposal and polyamine synthesis. As part of an enzymatic complex it contributes to the urea cycle which is important for the removal of excess nitrogen from the body. Arginase activity also influences the balance of nitric oxide synthesis as it competes with nitric oxide synthase for their common substrate L-arginine. This competition affects cellular functions which rely on nitric oxide signaling for developing proper vascular and immune responses.

Pathways

Arg2 is an important player in the urea cycle and the polyamine synthesis pathway. It works alongside the enzymes involved in these processes such as ornithine decarboxylase. Additionally Arg2 has a role in the nitric oxide regulation pathway modulating nitric oxide levels by limiting the availability of L-arginine for nitric oxide synthase. This makes Arg2 essential for metabolic processes and cellular communication.

Altered Arg2 expression has been associated with cardiovascular diseases and renal complications. For instance disruptions in Arg2 function can lead to endothelial dysfunction influencing conditions like atherosclerosis. Arg2 imbalances are also linked to kidney diseases possibly due to its critical role in ammonia detoxification and renal arginine metabolism. Its interaction with other proteins such as nitric oxide synthase further emphasizes its relevance in these health conditions.
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Specifications

Form

Liquid

Additional notes

ab97349 is purified using conventional chromatography techniques.

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General info

Function

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells (PubMed : 27745970). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed : 27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity). In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF-alpha production during gestation (PubMed : 28614294). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed : 22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed : 25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed : 23832324). Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed : 12859189).

Sequence similarities

Belongs to the arginase family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells (PubMed : 27745970). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed : 27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity). In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF-alpha production during gestation (PubMed : 28614294). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed : 22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed : 25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed : 23832324). Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed : 12859189).
See full target information ARG2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioorganic & medicinal chemistry 26:3939-3946 PubMed29914772

2018

Rational design of novel irreversible inhibitors for human arginase.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefeng Guo,Yiming Chen,Christopher T Seto
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