JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB123174

Recombinant Human ARH protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human ARH protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 308 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ARH, LDLRAP1, Low density lipoprotein receptor adapter protein 1, Autosomal recessive hypercholesterolemia protein

1 Images
SDS-PAGE - Recombinant Human ARH protein (His tag N-Terminus) (AB123174)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ARH protein (His tag N-Terminus) (AB123174)

15% SDS-PAGE analysis of 3ug ab123174.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q5SW96

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMDALKSAGRALIRSPSLAKQSWGGGGRHRKLPENWTDTRETLLEGMLFSLKYLGMTLVEQPKGEELSAAAIKRIVATAKASGKKLQKVTLKVSPRGIILTDNLTNQLIENVSIYRISYCTADKMHDKVFAYIAQSQHNQSLECHAFLCTKRKMAQAVTLTVAQAFKVAFEFWQVSKEEKEKRDKASQEGGDVLGARQDCTPPLKSLVATGNLLDLEETAKAPLSTVSANTTNMDEVPRPQALSGSSVVWELDDGLDEAFSRLAQSRTNPQVLDTGLTAQDMHYAQCLSPVDWDKPDSSGTEQDDLFSF","proteinLength":"Full Length","predictedMolecularWeight":"36.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":308,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q5SW96","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARH protein also known as autosomal recessive hypercholesterolemia protein plays an important role in the intracellular trafficking and endocytosis of low-density lipoprotein receptors (LDLR). ARH has a molecular mass of approximately 308 amino acids and is expressed primarily in the liver heart and adrenal gland. Its function is important in maintaining cholesterol homeostasis and influencing lipid metabolism by mediating receptor-ligand interactions.
Biological function summary

ARH facilitates the internalization of LDL particles by forming a complex with LDL receptors at the cell surface. This process helps in the uptake of cholesterol-rich lipoproteins an essential task for cell function. By binding adaptor proteins and clathrin ARH aids in creating pits that vesicles need during LDL endocytosis ensuring specific retrieval of receptors and ligands. This protein plays a role in maintaining cellular lipid balance and contributes to cholesterol regulation.

Pathways

ARH acts in the lipoprotein metabolism and cholesterol transport pathways by collaborating with other proteins. It interacts with LDLR and integrates into the clathrin-mediated endocytic pathway pivotal for cholesterol uptake and distribution within the body. It is associated with proteins such as LDLR and clathrin which perform key steps in lipoprotein processing and cholesterol homeostasis.

Alterations in ARH can lead to hypercholesterolemia a disorder characterized by elevated cholesterol levels in the blood and increased cardiovascular risk. ARH is associated with familial hypercholesterolemia reflecting its central role in cholesterol regulation. The protein's interaction with LDLR is important in this context as defects in this interaction result in impaired clearance of LDL from the bloodstream. Such disruptions emphasize the importance of ARH in lipid-related disorders and potential therapeutic targeting.
style
left-column

Specifications

Form

Liquid

Additional notes

ab123174 is purified by using conventional chromatography techniques.

style
left-column

General info

Function

Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytosis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression (By similarity).

style
left-column

Product protocols

style
left-column

Target data

Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytosis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression (By similarity).
See full target information LDLRAP1
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB116188

Recombinant Human GAS41 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GAS41 protein (His tag N-Terminus) (AB116188)

  • 0
  • 0
Proteins & Peptides

AB152790

Recombinant Human FIP protein

SDS-PAGE - Recombinant Human FIP protein (AB152790)

  • 0
  • 0
Proteins & Peptides

AB95933

Recombinant human XIAP protein

Functional Studies - Recombinant human XIAP protein (AB95933)

  • 0
  • 0
Proteins & Peptides

AB112374

Recombinant Human Tristetraprolin/TTP protein

SDS-PAGE - Recombinant Human Tristetraprolin/TTP protein (AB112374)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com