JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB125626

Recombinant human ARK5 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human ARK5 protein is a Human Full Length protein, in the 1 to 661 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

ARK5, KIAA0537, OMPHK1, NUAK1, NUAK family SNF1-like kinase 1, AMPK-related protein kinase 5, Omphalocele kinase 1

4 Images
Functional Studies - Recombinant human ARK5 protein (AB125626)
  • FuncS

Unknown

Functional Studies - Recombinant human ARK5 protein (AB125626)

The specific activity of ab125626 was determined to be 50 nmol/min/mg.

Functional Studies - Recombinant human ARK5 protein (AB125626)
  • FuncS

Supplier Data

Functional Studies - Recombinant human ARK5 protein (AB125626)

The specific activity of ARK5 (ab125626) was determined to be 54 nmol /min/mg as per activity assay protocol.

SDS-PAGE - Recombinant human ARK5 protein (AB125626)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human ARK5 protein (AB125626)

SDS-PAGE analysis of ab125626.

SDS-PAGE - Recombinant human ARK5 protein (AB125626)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human ARK5 protein (AB125626)

SDS PAGE analysis of ab125626

Key facts

Purity

>80% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, WB, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab125626 was determined to be 50 nmol/min/mg.

Accession

O60285

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
style
left-column

Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"122 kDa","actualMolecularWeight":null,"aminoAcidEnd":661,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O60285","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARK5 also known as NUAK2 is a protein kinase that supports various cellular processes. It weighs approximately 72 kDa and belongs to the AMP-activated protein kinase (AMPK)-related family. ARK5 shows high expression in the brain muscle and liver tissues. Its kinase activity regulates the phosphorylation of target proteins influencing cell survival and metabolism in different environments.
Biological function summary

ARK5 plays a role in aiding cellular adaptation to stress. It participates in a complex network involving energy sensing and signal transduction. As a serine/threonine kinase it influences processes like cell proliferation and glucose metabolism by interacting with other signaling molecules. ARK5 activation contributes to the regulation of cellular energy homeostasis ensuring cells can respond to energy deficiencies effectively.

Pathways

ARK5 integrates with key cellular pathways like the PI3K/AKT/mTOR pathway. This protein kinase activates in response to external signals like growth factors and cellular stress linking to AKT to modulate downstream effects. ARK5 also interacts with LKB1 a tumor suppressor that phosphorylates AMPK creating a connection between metabolic regulation and cell growth signals impacting cellular energy regulation and stress responses.

ARK5 involvement is noted in cancer and metabolic syndromes. Elevated ARK5 activity associates with tumor progression and metastasis particularly in breast cancer where it may correlate with poor prognosis. Additionally its role in glucose metabolism suggests a link to type 2 diabetes where dysregulated pathways including ARK5 influence insulin sensitivity. This connection often includes proteins like AKT which also participates in these disorders' pathophysiology.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1 : phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response : phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed : 25329316).

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Post-translational modifications

Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.. Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not dephosphorylated by the myosin PP1 complex when regulating its activity, due to the presence of PPP1R12A, which prevents myosin PP1 from dephosphorylating NUAK1. Phosphorylated by STK38L upon stimulation with IGF1.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1 : phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response : phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed : 25329316).
See full target information NUAK1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB80579

Anti-Tau antibody [TAU-5] - BSA and Azide free

Recombinant

Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (AB80579)

  • 5
  • 6
Primary Antibodies

AB210703

Anti-Tau (phospho S202 + T205) antibody [EPR20390]

20ul selling size|RabMAb|Recombinant|Trial Size

Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703)

  • 3
  • 3
Biochemicals

AB144820

WZ 4003, NUAK kinase inhibitor

Chemical Structure - WZ 4003, NUAK kinase inhibitor (AB144820)

  • 0
  • 0
Primary Antibodies

AB237594

Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (AB237594)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com