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AB113590

Recombinant Human ARL3 protein

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Recombinant Human ARL3 protein is a Human Full Length protein, in the 1 to 182 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ARFL3, ARL3, ADP-ribosylation factor-like protein 3

1 Images
SDS-PAGE - Recombinant Human ARL3 protein (AB113590)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ARL3 protein (AB113590)

15% SDS-PAGE analysis of ab113590 (3μg)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P36405

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMGLLSILRKLKSAPDQEVRILLLGLDNAGKTTLLKQLASEDISHITPTQGFNIKSVQSQGFKLNVWDIGGQRKIRPYWKNYFENTDILIYVIDSADRKRFEETGQELAELLEEEKLSCVPVLIFANKQDLLTAAPASEIAEGLNLHTIRDRVWQIQSCSALTGEGVQDGMNWVCKNVNAKKK","proteinLength":"Full Length","predictedMolecularWeight":"23 kDa","actualMolecularWeight":null,"aminoAcidEnd":182,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P36405","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL3 also known as ADP-ribosylation factor-like protein 3 is a GTP-binding protein involved in intracellular transport and signaling. It has a molecular mass of approximately 20 kDa. Scientists have observed its expression mainly in the cytoplasm but it also localizes to cilia centrosomes and in retina. ARL3 belongs to the Ras superfamily and exhibits strong GTPase activity. This feature allows ARL3 to regulate its active and inactive states switching between GTP-bound and GDP-bound forms.
Biological function summary

ARL3 plays a significant role in ciliary function and cellular signaling pathways. It forms a complex with ARL13B a protein essential for cilia development and function. This complex interacts with other factors to facilitate transport of lipidated proteins to ciliary membranes. By controlling lipid modification and transport ARL3 significantly influences the protein composition within cilia thereby impacting ciliary signaling.

Pathways

ARL3 integrates into critical processes including the phototransduction pathway and the sonic hedgehog pathway. Within these pathways it interacts with proteins like PDE6D and UNC119 which are involved in trafficking of specific proteins to the cilia. In phototransduction ARL3 modulates the proper localization of lipid-modified proteins necessary for visual signal transmission. Meanwhile in the sonic hedgehog pathway it plays a part in cilia-associated signaling essential for cellular communication and developmental processes.

ARL3 mutations have associations with retinitis pigmentosa a degenerative eye disease and Joubert syndrome a disorder characterized by developmental delays and brain malformations. ARL3 dysfunction disrupts ciliary transport and signaling leading to these conditions. In retinitis pigmentosa alterations in ARL3 affect interactions with proteins such as RPGR impacting photoreceptor function. For Joubert syndrome the improper function of ARL3 influences its relationship with proteins like ARL13B leading to abnormal ciliary signaling and subsequent developmental issues.
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Specifications

Form

Liquid

Additional notes

ab113590 was purified using conventional chromatography.

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General info

Function

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) (PubMed : 16525022, PubMed : 18588884). Required for normal cytokinesis and cilia signaling (PubMed : 22085962). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E (PubMed : 30269812). Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (PubMed : 22085962). Required for PKD1 : PKD2 complex targeting from the trans-Golgi network to the cilium (By similarity).

Sequence similarities

Belongs to the small GTPase superfamily. Arf family.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) (PubMed : 16525022, PubMed : 18588884). Required for normal cytokinesis and cilia signaling (PubMed : 22085962). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E (PubMed : 30269812). Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (PubMed : 22085962). Required for PKD1 : PKD2 complex targeting from the trans-Golgi network to the cilium (By similarity).
See full target information ARL3
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