Recombinant Human ARL5A protein is a Human Full Length protein, in the 1 to 179 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M G I L F T R I W R L F N H Q E H K V I I V G L D N A G K T T I L Y Q F S M N E V V H T S P T I G S N V E E I V I N N T R F L M W D I G G Q E S L R S S W N T Y Y T N T E F V I V V V D S T D R E R I S V T R E E L Y K M L A H E D L R K A G L L I F A N K Q D V K E C M T V A E I S Q F L K L T S I K D H Q W H I Q A C C A L T G E G L C Q G L E W M M S R L K I R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
ARFLP5, ARL5, ARL5A, ADP-ribosylation factor-like protein 5A
Recombinant Human ARL5A protein is a Human Full Length protein, in the 1 to 179 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9Y689-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00348% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M G I L F T R I W R L F N H Q E H K V I I V G L D N A G K T T I L Y Q F S M N E V V H T S P T I G S N V E E I V I N N T R F L M W D I G G Q E S L R S S W N T Y Y T N T E F V I V V V D S T D R E R I S V T R E E L Y K M L A H E D L R K A G L L I F A N K Q D V K E C M T V A E I S Q F L K L T S I K D H Q W H I Q A C C A L T G E G L C Q G L E W M M S R L K I R
- Accession
- Q9Y689
- Protein length
- Full Length
- Predicted molecular weight
- 23.3 kDa
- Amino acids
- 1 to 179
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab101943 is purified using conventional chromatography techniques.
General info
- Function
Lacks ADP-ribosylation enhancing activity.
- Sequence similarities
Belongs to the small GTPase superfamily. Arf family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARL5A also known as ADP-ribosylation factor-like protein 5A is a membrane-associated small GTPase with a molecular mass of approximately 21 kDa. It belongs to the ARF family involved in vesicular trafficking and signaling processes. ARL5A is expressed in various tissues with higher expression in brain and testis. The protein exhibits a GTP-binding with intrinsic GTPase activity playing an important role in the regulation of intracellular vesicle transport.
- Biological function summary
ARL5A functions as part of a larger molecular complex. The protein participates in endosomal trafficking processes and is associated with the Golgi apparatus. By modulating the dynamics of the Golgi-endosome transport system ARL5A influences vesicle budding and cargo sorting. It interacts with other proteins such as ARL5B to facilitate the formation and movement of vesicles in the cell.
- Pathways
This protein participates in the intracellular trafficking-related signaling pathways. It plays a part in the Wnt signaling pathway influencing vesicular movement between organelles. ARL5A interacts with proteins like GGA proteins which are adaptors that mediate cargo sorting and potentially links it with other important regulators in these pathways. This interaction allows cells to maintain homeostasis and properly respond to signaling events.
- Associated diseases and disorders
Mutant ARL5A has associations with neurodevelopmental disorders pointing to its significance in neural development. The protein's aberrant functions can disrupt normal cellular mechanisms contributing to disease states. It connects with proteins like ARF6 also a member of the ARF family which are implicated in the same regulatory networks. Understanding ARL5A's roles in these conditions could provide insights into potential therapeutic targets.
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1 product image
SDS-PAGE - Recombinant Human ARL5A protein (ab101943)
15% SDS-PAGE analysis of 3μg ab101943.
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