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AB276381

Recombinant Human ARMET/ARP protein (His tag)

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Recombinant Human ARMET/ARP protein (His tag) is a Human Full Length protein, in the 1 to 182 aa range, expressed in HEK 293 cells, with >96%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

ARMET, ARP, MANF, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMET

1 Images
SDS-PAGE - Recombinant Human ARMET/ARP protein (His tag) (AB276381)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ARMET/ARP protein (His tag) (AB276381)

SDS-PAGE analysis of ab276381

Key facts

Purity

>96% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P55145

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 100% PBS

storage-buffer

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Complementary Products

Primary Antibodies

AB316936

Anti-ARMET/ARP antibody [EPR29115-74] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody [EPR29115-74] - BSA and Azide free (AB316936)

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Primary Antibodies

AB200040

Anti-HAND2 antibody [EPR19451]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] (AB200040)

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Primary Antibodies

AB196622

Anti-HAND1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HAND1 antibody (AB196622)

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Primary Antibodies

AB28364

Anti-CD31 antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

  • 4
  • 160
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL","proteinLength":"Full Length","predictedMolecularWeight":"20 kDa","actualMolecularWeight":null,"aminoAcidEnd":182,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P55145","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARMET also known as Arp is a 19 kDa protein known for its role in cellular stress responses. It is alternatively named MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) in some studies. This protein is widely expressed including in the brain liver and pancreas reflecting its involvement across different cellular environments. ARMET/ARP interacts with the endoplasmic reticulum to address misfolded protein loads and support cell survival under stress.
Biological function summary

ARMET plays a significant role in maintaining cellular homeostasis especially under stress conditions. It does not form a larger complex but functions as a standalone factor that aids in the proper folding of proteins within the endoplasmic reticulum. Additionally ARMET has protective effects on dopaminergic neurons which are critical for neuromolecular processes. This protein has connections to cellular survival pathways acting as a neurotrophic factor to support neuron maintenance and health.

Pathways

ARMET is an important player in the unfolded protein response (UPR) and the neuroprotective pathways. In these pathways ARMET interacts with other proteins involved in protein folding and stress responses such as BiP/GRP78. These interactions help prevent apoptosis and promote cell survival by ensuring that cells can cope with endoplasmic reticulum stress. The ability of ARMET to enhance neuronal survival links it to pathways important for neuroprotection.

ARMET has been associated with neurodegenerative diseases such as Parkinson's disease and diabetes. In Parkinson's disease the survival-promoting effects of ARMET on dopaminergic neurons become vital potentially offering therapeutic targets. Connections between ARMET and diabetes surface through its regulation of beta-cell survival in the pancreas suggesting a role in maintaining insulin production and glucose homeostasis. In these diseases ARMET may interact with other proteins like proinsulin which holds significance for maintaining endocrine functions.
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Specifications

Form

Lyophilized

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General info

Function

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed : 12794311). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed : 18561914, PubMed : 22637475, PubMed : 29497057, PubMed : 36739529). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed : 22637475). Stabilizes HSPA5/BiP in its substrate-bound ADP state, which facilitates HSPA5/BiP incorporation into chaperone-client complexes during endoplasmic reticulum stress, its interaction with HSPA5/BiP inhibits ATP binding to HSPA5/BiP and subsequent nucleotide exchange (By similarity). As a result acts as a repressor of the unfolded protein response (UPR) pathway (By similarity). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed : 22637475). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed : 29497057). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed : 29497057). Essential for embryonic lung development (By similarity). Required for the correct postnatal temporal and structural development of splenic white pulp (By similarity). Required for the repair-associated myeloid response in skeletal muscle, acts as a regulator of phenotypic transition towards prorepair macrophages in response to muscle injury and as a result limits excessive proinflammatory signaling (By similarity). Represses RELA expression and therefore NF-kB signaling in the myocardium, as a result limits macrophage infiltration of injured tissue and M1 macrophage differentiation in response to myocardial injury (By similarity). Required for endochondral ossification in long bones and the skull during postnatal development (By similarity).

Sequence similarities

Belongs to the ARMET family.

Post-translational modifications

May contain sialic acid residues.

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Product protocols

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Target data

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed : 12794311). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed : 18561914, PubMed : 22637475, PubMed : 29497057, PubMed : 36739529). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed : 22637475). Stabilizes HSPA5/BiP in its substrate-bound ADP state, which facilitates HSPA5/BiP incorporation into chaperone-client complexes during endoplasmic reticulum stress, its interaction with HSPA5/BiP inhibits ATP binding to HSPA5/BiP and subsequent nucleotide exchange (By similarity). As a result acts as a repressor of the unfolded protein response (UPR) pathway (By similarity). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed : 22637475). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed : 29497057). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed : 29497057). Essential for embryonic lung development (By similarity). Required for the correct postnatal temporal and structural development of splenic white pulp (By similarity). Required for the repair-associated myeloid response in skeletal muscle, acts as a regulator of phenotypic transition towards prorepair macrophages in response to muscle injury and as a result limits excessive proinflammatory signaling (By similarity). Represses RELA expression and therefore NF-kB signaling in the myocardium, as a result limits macrophage infiltration of injured tissue and M1 macrophage differentiation in response to myocardial injury (By similarity). Required for endochondral ossification in long bones and the skull during postnatal development (By similarity).
See full target information MANF
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