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AB187418

Recombinant Human Arp3 protein (denatured) (His tag N-Terminus)

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Recombinant Human Arp3 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 418 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

ARP3, ACTR3, Actin-related protein 3, Actin-like protein 3

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SDS-PAGE - Recombinant Human Arp3 protein (denatured) (His tag N-Terminus) (AB187418)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Arp3 protein (denatured) (His tag N-Terminus) (AB187418)

15% SDS-PAGE analysis of 3 μg ab187418.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P61158

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS","proteinLength":"Full Length","predictedMolecularWeight":"50 kDa","actualMolecularWeight":null,"aminoAcidEnd":418,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61158","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arp3 also known as Actin-related protein 3 is an important component of the human cellular cytoskeleton machinery. It has a molecular mass of approximately 47 kDa and is ubiquitously expressed across various tissues. Arp3 participates in the nucleation and branching of actin filaments acting mechanically to control the structural organization of the cytoskeleton. This protein is highly conserved across species which shows its important role in fundamental cellular processes.
Biological function summary

Arp3 plays a significant role in cellular motility and morphology. It is a part of the Arp2/3 complex which consists of seven subunits that assemble to promote actin nucleation. This complex is central to the formation of branched actin networks which are necessary for processes such as cell movement endocytosis and vesicle trafficking. Through its mechanical action within the Arp2/3 complex Arp3 influences the dynamic rearrangement of the cytoskeleton necessary for cellular responses to external stimuli.

Pathways

Arp3 functions within the Wiskott-Aldrich syndrome protein (WASP) pathway and the Rho family of GTPases pathway. These pathways are important for regulating the actin cytoskeleton dynamics. The WASP pathway activates Arp2/3 complex triggering branched actin filament assembly. This process interacts with proteins like Cdc42 a member of the Rho GTPase family which further modulates cytoskeletal organization. Through these interactions Arp3 assists in coordinating actin-based processes critical to cell shape and movement.

Arp3 links to immune system problems and cancer progression. Mutations or dysregulation of the Arp2/3 complex including Arp3 can lead to immune deficiencies where cellular movement is impaired such as seen in Wiskott-Aldrich Syndrome. Furthermore aberrant Arp3 activity associates with cancer metastasis where altered cell motility plays a role in tumor spread. Connected proteins such as N-WASP often display interaction within these pathological contexts reinforcing Arp3's relevance in disease mechanisms.
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Specifications

Form

Liquid

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General info

Function

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947). Plays a role in ciliogenesis (PubMed : 20393563).

Sequence similarities

Belongs to the actin family. ARP3 subfamily.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947). Plays a role in ciliogenesis (PubMed : 20393563).
See full target information ACTR3
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Complementary Products

Primary Antibodies

AB151729

Anti-Arp3 antibody [EPR10428(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10428(B)] (AB151729)

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