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AB140555

Recombinant Human ARPC2 protein (His tag N-Terminus)

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Recombinant Human ARPC2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 300 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ARC34, PRO2446, ARPC2, Actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34 kDa subunit, p34-ARC

1 Images
SDS-PAGE - Recombinant Human ARPC2 protein (His tag N-Terminus) (AB140555)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ARPC2 protein (His tag N-Terminus) (AB140555)

15% SDS-PAGE analysis of ab140555 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O15144

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR","proteinLength":"Full Length","predictedMolecularWeight":"36.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":300,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15144","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARPC2 also known as p34-Arc is a critical component of the actin-related protein 2/3 complex (Arp2/3 complex) a protein complex that plays a major role in the regulation of the actin cytoskeleton. The molecular mass of ARPC2 is approximately 34 kDa. ARPC2 is highly expressed in several tissues including the brain lungs and the immune system. Its presence in diverse tissues suggests its fundamental role in various cellular processes.
Biological function summary

ARPC2 contributes to cell movement by promoting the nucleation of new actin filaments. It operates as part of the Arp2/3 complex which nucleates branches on pre-existing actin filaments a process necessary for the formation of a dynamic actin network within cells. The Arp2/3 complex where ARPC2 serves a structural and functional role is pivotal in cellular motility phagocytosis and endocytosis.

Pathways

ARPC2's involvement in cell shape and motility links it to signaling pathways such as the Rho GTPase pathway and the Wiskott-Aldrich syndrome protein (WASP) family member pathway. These pathways help modulate actin polymerization and cell movement. In these pathways ARPC2 works closely with proteins such as the Arp2/3 complex components and other regulatory elements like WASP which activates the complex to facilitate actin branching.

ARPC2's role in regulating actin dynamics connects it to conditions such as cancer and autoimmune diseases. Abnormal ARPC2 expression or function can lead to dysregulated cell movement and immune responses contributing to cancer metastasis and autoimmune diseases like systemic lupus erythematosus. In these disorders ARPC2 interacts with proteins that control immune function and cell migration highlighting its importance in maintaining normal physiological processes.
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Specifications

Form

Liquid

Additional notes

ab140555 was purified using conventional chromatography.

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General info

Function

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). Seems to contact the mother actin filament (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

Sequence similarities

Belongs to the ARPC2 family.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). Seems to contact the mother actin filament (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information ARPC2
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