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AB139214

Recombinant Human ARSG protein (denatured)

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Recombinant Human ARSG protein (denatured) is a Human Full Length protein, in the 17 to 525 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

KIAA1001, UNQ839/PRO1777, ARSG, Arylsulfatase G, ASG, N-sulfoglucosamine-3-sulfatase

1 Images
SDS-PAGE - Recombinant Human ARSG protein (denatured) (AB139214)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ARSG protein (denatured) (AB139214)

15% SDS-PAGE analysis of ab139214 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96EG1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSGFLYPLVDFCISGKTRGQKPNFVIILADDMGWGDLGANWAETKDTANLDKMASEGMRFVDFHAAASTCSPSRASLLTGRLGLRNGVTRNFAVTSVGGLPLNETTLAEVLQQAGYVTGIIGKWHLGHHGSYHPNFRGFDYYFGIPYSHDMGCTDTPGYNHPPCPACPQGDGPSRNLQRDCYTDVALPLYENLNIVEQPVNLSSLAQKYAEKATQFIQRASTSGRPFLLYVALAHMHVPLPVTQLPAAPRGRSLYGAGLWEMDSLVGQIKDKVDHTVKENTFLWFTGDNGPWAQKCELAGSVGPFTGFWQTRQGGSPAKQTTWEGGHRVPALAYWPGRVPVNVTSTALLSVLDIFPTVVALAQASLPQGRRFDGVDVSEVLFGRSQPGHRVLFHPNSGAAGEFGALQTVRLERYKAFYITGGARACDGSTGPELQHKFPLIFNLEDDTAEAVPLERGGAEYQAVLPEVRKVLADVLQDIANDNISSADYTQDPSVTPCCNPYQIACRCQAA","proteinLength":"Full Length","predictedMolecularWeight":"57 kDa","actualMolecularWeight":null,"aminoAcidEnd":525,"aminoAcidStart":17,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96EG1","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARSG protein also known as Arylsulfatase G functions as an essential enzyme in the breakdown of sulfate esters from glycosaminoglycans. It possesses a molecular mass of approximately 58 kDa and typically locates in the lysosomes of cells. ARSG expression occurs in a variety of tissues including the central nervous system liver and kidneys. Its enzymatic role is important for the catabolism of macromolecules which influences cellular recycling processes.
Biological function summary

The activity of ARSG influences the degradation of sulfated glycosaminoglycans which play significant roles in cellular signaling and structural integrity. It does not typically act within a multi-protein complex working individually to hydrolyze substrates within the lysosome. The balance maintained by ARSG ensures that cellular components are broken down at rate necessary for proper cellular function preventing harmful accumulation of materials that would otherwise disrupt cellular homeostasis.

Pathways

Four pathways are most notably affected by the activity of ARSG including the lysosomal degradation pathway. This protein works in concert with other sulfatases such as Arylsulfatase A (ARSA) to manage the breakdown and recycling of glycosaminoglycans. Proper functioning of these pathways under ARSG regulation also contributes to the overall health of the lysosomal storage system important for cellular metabolism and waste disposal.

Inefficient functioning of ARSG links to conditions such as multiple sulfatase deficiency (MSD) and late-infantile neuronal ceroid lipofuscinosis. Both disorders result from the accumulation of lysosomal storage materials due to defective breakdown processes. Arylsulfatase A (ARSA) also shares features with ARSG regarding MSD as both belong to the broader sulfatase enzyme family affected in this disorder. Understanding ARSG's role gives insight into potential therapeutic targets to address these lysosomal storage diseases.
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Specifications

Form

Liquid

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General info

Function

Displays arylsulfatase activity at acidic pH towards artificial substrates, such as p-nitrocatechol sulfate and also, but with a lower activity towards p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate (PubMed : 18283100, PubMed : 29300381). Catalyzes the hydrolysis of the 3-sulfate groups of the N-sulfo-D-glucosamine 3-O-sulfate units of heparin (PubMed : 22689975).

Sequence similarities

Belongs to the sulfatase family.

Post-translational modifications

N-glycosylated (PubMed:18283100). N-glycosylated with both high mannose and complex type sugars (By similarity).. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.. The 63-kDa precursor undergoes proteolytic processing in two steps, yielding two fragments in the first step (apparent molecular masses of 44 and 18 kDa) (PubMed:25135642). In the second step, the 44-kDa fragment is processed further to the 34- and 10-kDa chains. The 10-kDa chain is a cleavage product of the 44-kDa fragment but linked to the 18-kDa chain through a disulfide bridge (PubMed:25135642).

Subcellular localisation

Lysosome

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Product protocols

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Target data

Displays arylsulfatase activity at acidic pH towards artificial substrates, such as p-nitrocatechol sulfate and also, but with a lower activity towards p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate (PubMed : 18283100, PubMed : 29300381). Catalyzes the hydrolysis of the 3-sulfate groups of the N-sulfo-D-glucosamine 3-O-sulfate units of heparin (PubMed : 22689975).
See full target information ARSG
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