JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB229361

Recombinant Human ART4/DOK1 protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human ART4/DOK1 protein (His tag) is a Human Full Length protein, in the 47 to 285 aa range, expressed in Baculovirus infected insect cells, with >85%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

CD297, DO, DOK1, ART4, Ecto-ADP-ribosyltransferase 4, ADP-ribosyltransferase C2 and C3 toxin-like 4, Dombrock blood group carrier molecule, Mono(ADP-ribosyl)transferase 4, NAD(P)(+)--arginine ADP-ribosyltransferase 4, ARTC4

1 Images
SDS-PAGE - Recombinant Human ART4/DOK1 protein (His tag) (AB229361)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ART4/DOK1 protein (His tag) (AB229361)

15% SDS-PAGE analysis of 3 μg ab229361.

MW 28-40 kDa (SDS-PAGE under reducing conditions).

Key facts

Purity

>85% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q93070

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as ART4
style
left-column

Sequence info

[{"sequence":"ADPSEVAIKIDFDFAPGSFDDQYQGCSKQVMEKLTQGDYFTKDIEAQKNYFRMWQKAHLAWLNQGKVLPQNMTTTHAVAILFYTLNSNVHSDFTRAMASVARTPQQYERSFHFKYLHYYLTSAIQLLRKDSIMENGTLCYEVHYRTKDVHFNAYTGATIRFGQFLSTSLLKEEAQEFGNQTLFTIFTCLGAPVQYFSLKKEVLIPPYELFKVINMSYHPRGDWLQLRSTGNLSTYNCQLLKAHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"28.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":285,"aminoAcidStart":47,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"Q93070","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ART4 also known as CD297 and DOK1 play critical roles in cell signaling. ART4 belongs to the ADP-ribosyltransferase family and is a GPI-anchored protein with a molecular mass of about 30 kDa while DOK1 is an adapter protein with a molecular mass around 50 kDa. ART4 is expressed on erythrocytes and some lymphocyte subpopulations and DOK1 is expressed in various tissues most notably in hematopoietic cells.
Biological function summary

The two proteins engage in distinct functions within cellular mechanisms. ART4 participates in cell communication and receptor signaling on the cell surface. DOK1 acts as a modulator of signal transduction being part of signalosome complexes. Their interaction facilitates proper signaling which influences cell growth and immune responses.

Pathways

Proteins like ART4 and DOK1 play relevant roles in regulating immune cell function and hematopoiesis. ART4 is involved in the complement pathway while DOK1 connects to the Ras-MAPK signaling pathway. These proteins position themselves alongside major signaling molecules like SHC and GRB2 which participate in signal transduction cascades.

ART4 and DOK1 have implications in autoimmune diseases and leukemia. In autoimmune disorders abnormal ART4 expression alters immune responses. In leukemia DOK1's regulation of cell proliferation links it to leukemia progression correlating with proteins such as ABL1 in signaling abnormalities.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified

style
left-column

General info

Sequence similarities

Belongs to the Arg-specific ADP-ribosyltransferase family.

style
left-column

Product protocols

style
left-column

Target data

See full target information ART4
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB316501

Recombinant Human CD3 Epsilon Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CD3 Epsilon Protein Standard (His tag) (AB316501)

  • 0
  • 0
Primary Antibodies

AB108930

Anti-Tenascin C antibody [EPR4219]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)

  • 4
  • 16
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB977

Anti-E2 tag antibody [5E11]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (AB977)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com