Recombinant Human ART4/DOK1 protein (His tag) is a Human Full Length protein, in the 47 to 285 aa range, expressed in Baculovirus infected insect, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P S E V A I K I D F D F A P G S F D D Q Y Q G C S K Q V M E K L T Q G D Y F T K D I E A Q K N Y F R M W Q K A H L A W L N Q G K V L P Q N M T T T H A V A I L F Y T L N S N V H S D F T R A M A S V A R T P Q Q Y E R S F H F K Y L H Y Y L T S A I Q L L R K D S I M E N G T L C Y E V H Y R T K D V H F N A Y T G A T I R F G Q F L S T S L L K E E A Q E F G N Q T L F T I F T C L G A P V Q Y F S L K K E V L I P P Y E L F K V I N M S Y H P R G D W L Q L R S T G N L S T Y N C Q L L K A H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
CD297, DO, DOK1, ART4, Ecto-ADP-ribosyltransferase 4, ADP-ribosyltransferase C2 and C3 toxin-like 4, Dombrock blood group carrier molecule, Mono(ADP-ribosyl)transferase 4, NAD(P)(+)--arginine ADP-ribosyltransferase 4, ARTC4
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Recombinant Human ART4/DOK1 protein (His tag) is a Human Full Length protein, in the 47 to 285 aa range, expressed in Baculovirus infected insect, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q93070-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P S E V A I K I D F D F A P G S F D D Q Y Q G C S K Q V M E K L T Q G D Y F T K D I E A Q K N Y F R M W Q K A H L A W L N Q G K V L P Q N M T T T H A V A I L F Y T L N S N V H S D F T R A M A S V A R T P Q Q Y E R S F H F K Y L H Y Y L T S A I Q L L R K D S I M E N G T L C Y E V H Y R T K D V H F N A Y T G A T I R F G Q F L S T S L L K E E A Q E F G N Q T L F T I F T C L G A P V Q Y F S L K K E V L I P P Y E L F K V I N M S Y H P R G D W L Q L R S T G N L S T Y N C Q L L K A H H H H H H
- Accession
- Q93070
- Protein length
- Full Length
- Predicted molecular weight
- 28.8 kDa
- Amino acids
- 47 to 285
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Sequence similarities
Belongs to the Arg-specific ADP-ribosyltransferase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as ART4
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ART4 also known as CD297 and DOK1 play critical roles in cell signaling. ART4 belongs to the ADP-ribosyltransferase family and is a GPI-anchored protein with a molecular mass of about 30 kDa while DOK1 is an adapter protein with a molecular mass around 50 kDa. ART4 is expressed on erythrocytes and some lymphocyte subpopulations and DOK1 is expressed in various tissues most notably in hematopoietic cells.
- Biological function summary
The two proteins engage in distinct functions within cellular mechanisms. ART4 participates in cell communication and receptor signaling on the cell surface. DOK1 acts as a modulator of signal transduction being part of signalosome complexes. Their interaction facilitates proper signaling which influences cell growth and immune responses.
- Pathways
Proteins like ART4 and DOK1 play relevant roles in regulating immune cell function and hematopoiesis. ART4 is involved in the complement pathway while DOK1 connects to the Ras-MAPK signaling pathway. These proteins position themselves alongside major signaling molecules like SHC and GRB2 which participate in signal transduction cascades.
- Associated diseases and disorders
ART4 and DOK1 have implications in autoimmune diseases and leukemia. In autoimmune disorders abnormal ART4 expression alters immune responses. In leukemia DOK1's regulation of cell proliferation links it to leukemia progression correlating with proteins such as ABL1 in signaling abnormalities.
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1 product image
SDS-PAGE - Recombinant Human ART4/DOK1 protein (His tag) (ab229361)
15% SDS-PAGE analysis of 3 μg ab229361.
MW 28-40 kDa (SDS-PAGE under reducing conditions).
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