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AB271650

Recombinant human ARTD10 protein (Tagged)

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Recombinant human ARTD10 protein (Tagged) is a Human Fragment protein, in the 805 to 1025 aa range, expressed in Baculovirus infected Sf9 cells, with >86%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Protein mono-ADP-ribosyltransferase PARP10, ADP-ribosyltransferase diphtheria toxin-like 10, Poly [ADP-ribose] polymerase 10, ARTD10, PARP-10, PARP10

2 Images
Functional Studies - Recombinant human ARTD10 protein (Tagged) (AB271650)
  • FuncS

Unknown

Functional Studies - Recombinant human ARTD10 protein (Tagged) (AB271650)

Specific activity of ab271650.

SDS-PAGE - Recombinant human ARTD10 protein (Tagged) (AB271650)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human ARTD10 protein (Tagged) (AB271650)

SDS-PAGE analysis of ab271650.

Key facts

Purity

>86% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Active

Accession

Q53GL7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"GPTLAGQTLKGPWNNLERLAENTGEFQEVVRAFYDTLDAARSSIRVVRVERVSHPLLQQQYELYRERLLQRCERRPVEQVLYHGTTAPAVPDICAHGFNRSFCGRNATVYGKGVYFARRASLSVQDRYSPPNADGHKAVFVARVLTGDYGQGRRGLRAPPLRGPGHVLLRYDSAVDCICQPSIFVIFHDTQALPTHLITCEHVPRASPDDPSGLPGRSPDT","proteinLength":"Fragment","predictedMolecularWeight":"27 kDa","actualMolecularWeight":null,"aminoAcidEnd":1025,"aminoAcidStart":805,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q53GL7","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ARTD10 also known as PARP10 is a member of the poly(ADP-ribose) polymerase (PARP) family. It exhibits a mass of approximately 100 kDa. ARTD10 acts mechanically as mono-ADP-ribosyltransferase which modifies target proteins by adding ADP-ribose units. This specific post-translational modification controls protein function and interactions. ARTD10 expression occurs mostly in the cytoplasm and nucleus across different cell types indicating its versatile functional roles.
Biological function summary

ARTD10 influences several cellular processes including transcriptional regulation and cell cycle progression. It functions independently without being part of a stable protein complex but interacts with other nuclear and cytoplasmic proteins to exert its role. This protein is involved in modulating cellular responses to DNA damage and stress impacting cellular homeostasis and metabolism. Its interaction patterns highlight its significance in cellular regulatory mechanisms.

Pathways

ARTD10 is an active participant in DNA repair and stress response pathways. It is closely linked with the NF-kB signaling pathway where it modulates inflammation and cell survival outcomes. ARTD10 also connects with proteins like PARP1 sharing a broader role in the maintenance of genomic integrity and response to DNA insults. These pathways underpin the critical mechanisms by which cells manage and adapt to damages and maintain cellular viability.

ARTD10's dysregulation associates with certain cancers and inflammatory conditions. Aberrant activity of ARTD10 impacts cellular proliferation and apoptosis linking it with tumorigenesis. Inflammation-related disorders also see a correlation with altered ARTD10 activity. This protein remains related to key proteins like NF-kB in understanding its role in these diseases as they collectively influence inflammatory and tumor progression pathways.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins (PubMed : 18851833, PubMed : 23332125, PubMed : 23474714, PubMed : 25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 18851833). Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity (PubMed : 23332125). Involved in translesion DNA synthesis in response to DNA damage via its interaction with PCNA (PubMed : 24695737).

Sequence similarities

Belongs to the ARTD/PARP family.

Post-translational modifications

Stimulated through its phosphorylation by CDK2 (PubMed:16455663). Acquires CDK-dependent phosphorylation through late-G1 to S phase, and from prometaphase to cytokinesis in the nucleolar organizing regions (PubMed:16455663). Phosphorylation is suppressed in growth-arrested cells (PubMed:16455663).. Auto-mono-ADP-ribosylated on glutamate and lysine residues.

Subcellular localisation

Nucleus

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Product protocols

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Target data

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins (PubMed : 18851833, PubMed : 23332125, PubMed : 23474714, PubMed : 25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 18851833). Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity (PubMed : 23332125). Involved in translesion DNA synthesis in response to DNA damage via its interaction with PCNA (PubMed : 24695737).
See full target information PARP10
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