Recombinant Human ASAH1 protein (His tag) is a Human Full Length protein, in the 22 to 395 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
Q H A P P W T E D C R K S T Y P P S G P T Y R G A V P W Y T I N L D L P P Y K R W H E L M L D K A P V L K V I V N S L K N M I N T F V P S G K I M Q V V D E K L P G L L G N F P G P F E E E M K G I A A V T D I P L G E I I S F N I F Y E L F T I C T S I V A E D K K G H L I H G R N M D F G V F L G W N I N N D T W V I T E Q L K P L T V N L D F Q R N N K T V F K A S S F A G Y V G M L T G F K P G L F S L T L N E R F S I N G G Y L G I L E W I L G K K D V M W I G F L T R T V L E N S T S Y E E A K N L L T K T K I L A P A Y F I L G G N Q S G E G C V I T R D R K E S L D V Y E L D A K Q G R W Y V V Q T N Y D R W K H P F F L D D R R T P A K M C L N R T S Q E N I S F E T M Y D V L S T K P V L N K L T V Y T T L I D V T K G Q F E T Y L R D C P D P C I G W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH (PubMed:10610716, PubMed:7744740, PubMed:15655246, PubMed:11451951). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:10610716). Has a higher catalytic efficiency towards C12-ceramides versus other ceramides (PubMed:7744740, PubMed:15655246). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:12764132, PubMed:12815059). For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used (PubMed:12764132). Has also an N-acylethanolamine hydrolase activity (PubMed:15655246). By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes (PubMed:17713573). Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity). By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis (PubMed:22261821).Isoform 2May directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity.
Alternative names
Acid ceramidase, AC, ACDase, Acid CDase, Acylsphingosine deacylase, N-acylethanolamine hydrolase ASAH1, N-acylsphingosine amidohydrolase, Putative 32 kDa heart protein, PHP32, HSD33, HSD-33, ASAH, ASAH1
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Recombinant Human ASAH1 protein (His tag) is a Human Full Length protein, in the 22 to 395 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q13510-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
Q H A P P W T E D C R K S T Y P P S G P T Y R G A V P W Y T I N L D L P P Y K R W H E L M L D K A P V L K V I V N S L K N M I N T F V P S G K I M Q V V D E K L P G L L G N F P G P F E E E M K G I A A V T D I P L G E I I S F N I F Y E L F T I C T S I V A E D K K G H L I H G R N M D F G V F L G W N I N N D T W V I T E Q L K P L T V N L D F Q R N N K T V F K A S S F A G Y V G M L T G F K P G L F S L T L N E R F S I N G G Y L G I L E W I L G K K D V M W I G F L T R T V L E N S T S Y E E A K N L L T K T K I L A P A Y F I L G G N Q S G E G C V I T R D R K E S L D V Y E L D A K Q G R W Y V V Q T N Y D R W K H P F F L D D R R T P A K M C L N R T S Q E N I S F E T M Y D V L S T K P V L N K L T V Y T T L I D V T K G Q F E T Y L R D C P D P C I G W
- Accession
- Protein length
Full Length
- Predicted molecular weight
58.7 kDa
- Amino acids
22 to 395
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH (PubMed:10610716, PubMed:7744740, PubMed:15655246, PubMed:11451951). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:10610716). Has a higher catalytic efficiency towards C12-ceramides versus other ceramides (PubMed:7744740, PubMed:15655246). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:12764132, PubMed:12815059). For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used (PubMed:12764132). Has also an N-acylethanolamine hydrolase activity (PubMed:15655246). By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes (PubMed:17713573). Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity). By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis (PubMed:22261821).
- Sequence similarities
Belongs to the acid ceramidase family.
- Post-translational modifications
N-glycosylated.
- Subcellular localisation
Lysosome, Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASAH1 also known as acid ceramidase or alkaline ceramidase is an enzyme that plays a role in the hydrolysis of ceramides into sphingosine and free fatty acids. It has a mass of approximately 55 kDa. This enzyme is primarily found in lysosomes cellular organelles that manage waste. ASAH1 is expressed in various tissues including liver heart brain and skin illustrating its widespread distribution in the body.
- Biological function summary
ASAH1 influences several important cellular processes by controlling the levels of ceramide and sphingosine. These lipids are not just structural components of membranes; they participate actively in cell signaling. ASAH1 exists as part of a larger complex that includes other enzymes involved in sphingolipid metabolism. Changes in ceramide levels influence apoptosis and cell proliferation indicating ASAH1's role as a regulator in these processes.
- Pathways
ASAH1 is central to the sphingolipid metabolism pathway. This pathway is interconnected with the apoptosis signaling pathway. By converting ceramide to sphingosine ASAH1 links the complex ceramide/sphingosine balance affecting cell survival and death decisions. It interacts with other enzymes such as sphingosine kinase 1 and ceramide synthases which further maintain the dynamic regulation between sphingosine and ceramide levels.
- Associated diseases and disorders
ASAH1 is linked to conditions such as Farber disease and spinal muscular atrophy. These disorders result from dysfunctional ceramide metabolism causing cellular and systemic impacts due to excessive ceramide accumulation. Proteins like acid sphingomyelinase are related to ASAH1's role in these diseases as both contribute to sphingolipid balance in cells. Understanding ASAH1 and its pathways provides insights into potential therapeutic targets for these sphingolipid-related disorders.
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SDS-PAGE - Recombinant Human ASAH1 protein (His tag) (ab226446)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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