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Recombinant Human ASAM protein (His tag) is a Human Fragment protein, in the 1 to 233 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human ASAM protein (His tag) (AB276317), expandable thumbnail

Key facts

Purity

>97% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M S L L L L L L L V S Y Y V G T L G T H T E I K R V A E E K V T L P C H H Q L G L P E K D T L D I E W L L T D N E G N Q K V V I T Y S S R H V Y N N L T E E Q K G R V A F A S N F L A G D A S L Q I E P L K P S D E G R Y T C K V K N S G R Y V W S H V I L K V L V R P S K P K C E L E G E L T E G S D L T L Q C E S S S G T E P I V Y Y W Q R I R E K E G E D E R L P P K S R I D Y N H P G R V L L Q N L T M S Y S G L Y Q C T A G N E A G K E S C V V R V T V Q Y V Q S I G M

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

May be involved in the cell-cell adhesion. May play a role in adipocyte differentiation and development of obesity. Is required for normal small intestine development.

Alternative names

Recommended products

Recombinant Human ASAM protein (His tag) is a Human Fragment protein, in the 1 to 233 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>97% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE

Accession
Q9H6B4-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 100% PBS

Sequence info

Amino acid sequence

M S L L L L L L L V S Y Y V G T L G T H T E I K R V A E E K V T L P C H H Q L G L P E K D T L D I E W L L T D N E G N Q K V V I T Y S S R H V Y N N L T E E Q K G R V A F A S N F L A G D A S L Q I E P L K P S D E G R Y T C K V K N S G R Y V W S H V I L K V L V R P S K P K C E L E G E L T E G S D L T L Q C E S S S G T E P I V Y Y W Q R I R E K E G E D E R L P P K S R I D Y N H P G R V L L Q N L T M S Y S G L Y Q C T A G N E A G K E S C V V R V T V Q Y V Q S I G M

Accession

Q9H6B4

Protein length

Fragment

Predicted molecular weight

25.8 kDa

Amino acids

1 to 233

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Lyophilized

General info

Function

May be involved in the cell-cell adhesion. May play a role in adipocyte differentiation and development of obesity. Is required for normal small intestine development.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ASAM protein also known as the Adipocyte plasma Membrane Associated Protein (APPL1) is a transmembrane protein with a mass around 34 kDa. It is highly expressed in adipose tissue and endothelial cells but appears across a range of tissues. The ASAM protein plays a role in adhesion processes by facilitating cell-cell interactions and is important in maintaining cellular architecture. It interacts with multiple binding partners suggesting diverse functionality and involvement in cellular signaling pathways.

Biological function summary

The ASAM protein has significant roles in cellular adhesion and signaling. As part of the plasma membrane it modulates interactions between cells and their environment contributing to tissue development and repair. It is not typically known to be part of a larger complex but its ability to bind with other proteins highlights its versatility in cellular regulation. Researchers understand it better in the context of cell structural dynamics and communication processes.

Pathways

Research identifies the ASAM protein as a player in the insulin signaling pathway and the cellular adhesion pathway. In insulin signaling it interacts with proteins such as IGF1R and PI3K connecting it to metabolic regulation and homeostasis. The cellular adhesion pathway finds ASAM working with cadherins and integrins which emphasizes its function in cell communication and structural integrity. Its engagement in these pathways indicates its influence on metabolic processes and cellular response mechanisms.

Associated diseases and disorders

The ASAM protein shows relevance in conditions like diabetes and obesity. Its involvement in insulin signaling aligns it with metabolic disorders where dysregulation affects glucose uptake and lipid metabolism. Histological studies indicate ASAM interactions with other proteins like FAK and AMPK in the context of these diseases. This connection suggests its potential as a biomarker for metabolic syndromes providing insight into disease development and therapeutic target exploration.

Product promise

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1 product image

  • SDS-PAGE - Recombinant Human ASAM protein (His tag) (ab276317), expandable thumbnail

    SDS-PAGE - Recombinant Human ASAM protein (His tag) (ab276317)

    SDS-PAGE analysis of ab276317

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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