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AB84211

Recombinant Human ASF1A protein (His tag N-Terminus)

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Recombinant Human ASF1A protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

CGI-98, HSPC146, ASF1A, Histone chaperone ASF1A, Anti-silencing function protein 1 homolog A, CCG1-interacting factor A, hAsf1, hAsf1a, CIA, hCIA

1 Images
SDS-PAGE - Recombinant Human ASF1A protein (AB84211)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ASF1A protein (AB84211)

3µg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9Y294

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Endotoxin level: < 1.0 EU per 1 μg of protein (determined by LAL method).
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y294","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASF1A also known as Anti-silencing Function 1A is a histone chaperone protein with a molecular weight of approximately 22 kDa. This protein aids in the assembly and disassembly of chromatin by facilitating the transfer of histones H3 and H4 to nascent DNA strands during replication and repair processes. ASF1A is expressed in various tissues including high levels in actively dividing cells such as those in the testes and bone marrow. It plays a critical role in maintaining cellular proliferative capacity.
Biological function summary

ASF1A is part of the chromatin remodeling machinery ensuring proper chromatin dynamics and genome integrity. It acts in conjunction with other proteins such as CAF-1 and HIRA as part of the histone deposition process. ASF1A contributes to DNA replication repair and transcription regulation. Through interaction with these complexes ASF1A supports the replacement of histones during S-phase and post-replication repair which is essential for cell cycle progression and genome stability.

Pathways

ASF1A has significant engagement in pathways associated with DNA replication and chromatin modification. It operates within the DNA damage checkpoint pathway where it collaborates with proteins like ATR and CHK1 to ensure proper DNA damage response and cell cycle control. ASF1A also associates with the replication fork protection pathway important for the stabilization of halted replication forks and prevention of genomic instability.

ASF1A has connections to certain cancers and neurodegenerative disorders. Its overexpression or dysregulation links to ovarian and lung cancers due to its role in promoting uncontrolled cell proliferation. ASF1A's involvement in ensuring DNA replication fidelity implicates it in disorders like Alzheimer's disease where genomic instability and improper cell division may play a role. Proteins such as p53 and BRCA1 which are key regulators in these conditions show indirect or direct interactions with ASF1A highlighting its importance in disease pathophysiology.
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Specifications

Form

Liquid

Additional notes

ab84211 is purified using conventional chromatography techniques.

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General info

Function

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed : 10759893, PubMed : 11897662, PubMed : 12842904, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251, PubMed : 21454524). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198). Promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks : acts by mediating histone replacement at DSBs, leading to recruitment of the MMS22L-TONSL complex and subsequent loading of RAD51 (PubMed : 29478807). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4) : specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' and acetylation at 'Lys-14' (H3K9me1K14ac) marks, and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed : 21454524, PubMed : 29408485). Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit (PubMed : 15621527).

Sequence similarities

Belongs to the ASF1 family.

Post-translational modifications

Phosphorylated by TLK1 and TLK2 (PubMed:11470414, PubMed:20016786, PubMed:35136069). Highly phosphorylated in S-phase and at lower levels in M-phase (PubMed:11470414). TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation (PubMed:20016786). Phosphorylation at Ser-192 by PRKDC in response to DNA damage promotes the histone chaperone activity and ability to replace histones at double-strand breaks (DSBs) at stalled or collapsed replication forks, leading to RAD51 recruitment (PubMed:29478807).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed : 10759893, PubMed : 11897662, PubMed : 12842904, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251, PubMed : 21454524). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198). Promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks : acts by mediating histone replacement at DSBs, leading to recruitment of the MMS22L-TONSL complex and subsequent loading of RAD51 (PubMed : 29478807). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4) : specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' and acetylation at 'Lys-14' (H3K9me1K14ac) marks, and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed : 21454524, PubMed : 29408485). Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit (PubMed : 15621527).
See full target information ASF1A
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