Recombinant Human ASF1b protein (His tag C-Terminus)
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(1 Publication)
Recombinant Human ASF1b protein (His tag C-Terminus) is a Human Full Length protein, in the 1 to 202 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, hAsf1, hAsf1b, CIA-II, hCIA-II, ASF1B
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human ASF1b protein (His tag C-Terminus) (AB130033)
ab130033 at 3 μg analysed by 15% SDS-PAGE.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein assists in the maintenance and regulation of chromatin structure impacting DNA replication and transcription. ASF1b interacts with histone H3/H4 complexes and often works in coordination with CAF-1 forming a multi-protein complex important for nucleosome assembly during DNA replication. Through these interactions ASF1b plays a role in both maintaining genomic stability and facilitating proper cell cycle progression.
Pathways
ASF1b contributes to the DNA replication and repair pathways required for accurate duplication of genetic material. In the DNA damage response ASF1b offers histone supply critical for chromatin structure restoration post-repair. It also interacts with proteins like ATR and CHK1 aiding in the checkpoint response to DNA damage to prevent genomic instability. However its precise regulation within these pathways can have significant cellular consequences related to genome integrity.
Specifications
Form
Liquid
Additional notes
ab130033 was purified by using conventional chromatography techniques.
General info
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251, PubMed : 21454524, PubMed : 26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4) : specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed : 20953179, PubMed : 21454524, PubMed : 26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251). Required for gonad development (PubMed : 12842904).
Sequence similarities
Belongs to the ASF1 family.
Post-translational modifications
Phosphorylated by TLK1 and TLK2.
Subcellular localisation
Nucleus
Target data
Publications (1)
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Molecular cell 83:1075-1092.e9 PubMed36868228
2023
Applications
Unspecified application
Species
Unspecified reactive species
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