Recombinant Human ASH1L protein is a Human Fragment protein, in the 2433 to 2548 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M H H H H H H E V A R A A R L A Q I F K E I C D G I I S Y K D S S R Q A L A A P L L N L P P K K K N A D Y Y E K I S D P L D L I T I E K Q I L T G Y Y K T V E A F D A D M L K V F R N A E K Y Y G R K S P V G R D V C R L R K A Y Y N A R H E A S A Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Histone methyltransferase specifically trimethylating 'Lys-36' of histone H3 forming H3K36me3 (PubMed:21239497). Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). The physiological significance of the H3K9me1 activity is unclear (By similarity).
Alternative names
KIAA1420, KMT2H, ASH1L, Histone-lysine N-methyltransferase ASH1L, ASH1-like protein, Absent small and homeotic disks protein 1 homolog, Lysine N-methyltransferase 2H, huASH1
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Recombinant Human ASH1L protein is a Human Fragment protein, in the 2433 to 2548 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9NR48-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Tris HCl, 0.63% Sodium chloride, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M H H H H H H E V A R A A R L A Q I F K E I C D G I I S Y K D S S R Q A L A A P L L N L P P K K K N A D Y Y E K I S D P L D L I T I E K Q I L T G Y Y K T V E A F D A D M L K V F R N A E K Y Y G R K S P V G R D V C R L R K A Y Y N A R H E A S A Q
- Accession
- Q9NR48
- Protein length
- Fragment
- Predicted molecular weight
- 14.23 kDa
- Amino acids
- 2433 to 2548
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Histone methyltransferase specifically trimethylating 'Lys-36' of histone H3 forming H3K36me3 (PubMed:21239497). Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). The physiological significance of the H3K9me1 activity is unclear (By similarity).
- Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
- Post-translational modifications
Methylated at Gln-1220 by N6AMT1.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as KMT2H / ASH1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASH1L also known as Absent Small or Homeotic-Like 1 is a protein-coding gene that functions as a histone lysine methyltransferase. This enzyme has a large mass of around 296 kDa and is mainly expressed in the human brain and the testes. It is located in the euchromatic region and participates in depositing methyl groups on lysine residues of histones which influences chromatin structure and gene expression. Due to its ability to modify chromatin ASH1L plays a significant role in regulating transcription.
- Biological function summary
ASH1L contributes to various processes involving transcriptional regulation and chromatin modification. This protein functions as part of the Set1/Ash1 complex where it influences gene expression by specifically methylating histone H3 at lysine 36 (H3K36). The methylation of this residue is associated with active transcription and is important for the proper regulation of genes involved in development and differentiation. ASH1L-mediated modifications thereby facilitate the global maintenance of the active chromatin state essential for cellular processes.
- Pathways
ASH1L is engaged in pathways related to histone modification and transcriptional regulation. It functions alongside proteins in the Wnt signaling pathway which plays a vital role in regulating tissue-specific transcription programs. ASH1L can alternatively interact with other histone methyltransferases like the NSD family which share similar functions in chromatin dynamics and are involved in transcription elongation and chromatin remodeling.
- Associated diseases and disorders
ASH1L has been associated with developmental disorders and certain cancers. Its function in epigenetic regulation makes it significant in neurodevelopmental disorders where disruptions in its expression or function can lead to conditions such as autism spectrum disorders. Additionally ASH1L's misregulation has been linked to leukemia where it interacts with mixed-lineage leukemia (MLL) proteins due to its role in regulating gene expression involved in cell proliferation.
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1 product image
SDS-PAGE - Recombinant Human ASH1L protein (ab195166)
4-20% SDS-PAGE using 2.7 μg ab195166 (lane 1). Lane 2 is protein marker.
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