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AB114681

Recombinant Human ASIC1 protein (GST tag N-Terminus)

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Recombinant Human ASIC1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 528 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

ACCN2, BNAC2, ASIC1, Acid-sensing ion channel 1, Brain sodium channel 2

1 Images
SDS-PAGE - Recombinant Human ASIC1 protein (GST tag N-Terminus) (AB114681)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ASIC1 protein (GST tag N-Terminus) (AB114681)

ab114681 analyzed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P78348

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB240896

Anti-ASIC1 antibody [S271-44]

Immunocytochemistry/ Immunofluorescence - Anti-ASIC1 antibody [S271-44] (AB240896)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as ACCN2.
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Sequence info

[{"sequence":"MELKAEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFHYHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEVCSAEDFKVVFTRYGKCYTFNSGRDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVACQEQRLIYLPPPWGTCKAVTMDSDLDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHKLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC","proteinLength":"Full Length","predictedMolecularWeight":"84.15 kDa","actualMolecularWeight":null,"aminoAcidEnd":528,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P78348","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ASIC1 or acid-sensing ion channel 1 is also known as ASIC or ACCN2. It is part of a group of proton-gated cation channels. ASIC1 has a molecular mass of approximately 60 kDa. This ion channel plays a role in the detection of acidosis where it mediates proton influx across cell membranes affecting changes in membrane potential. ASIC1 is expressed in neurons of the central and peripheral nervous systems with particular abundance in the brain. Its localization to regions such as the amygdala suggests a possible role in processing emotions and pain perception.
Biological function summary

This ion channel aids in neuronal response to pH drops serving as a sensor for acidification. The ASIC1 subunit can form homotrimers or heterotrimers with other ASIC subunits which impacts the ion conductance properties. This protein's activity affects synaptic plasticity and is involved in both short-term and long-term modulation of neuronal circuits. Changes in its activity can influence neurotransmission and has implications in neurological functionality.

Pathways

ASIC1 is part of the neurophysiological signaling pathways affecting synaptic transmission. It interacts with other proteins like ASIC2 and ASIC3 being implicated in the modulation of sensory perception and pain. Involvement in pathways like the pain-transducing signaling pathway places ASIC1 in a context where it can regulate nociceptive signals. It participates in ion flux alterations that contribute to neuronal excitability and synaptic strength.

ASIC1 has connections to neurodegenerative conditions and pain syndromes. It is implicated in the pathophysiology of disorders such as ischemia where acidosis occurs during stress and tissue damage and chronic pain conditions. ASIC1 interacts with proteins such as the NMDA receptor in the context of these diseases suggesting a role in excitotoxicity. Dysfunction or altered regulation of ASIC1 may exacerbate neurodegeneration or chronic pain symptoms highlighting its potential as a therapeutic target.
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Specifications

Form

Liquid

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General info

Function

Forms voltage-independent, pH-gated trimeric sodium channels that act as postsynaptic excitatory receptors in the nervous system, playing a crucial role in regulating synaptic plasticity, learning, and memory (PubMed : 21036899, PubMed : 32915133, PubMed : 34319232). Upon extracellular pH drop this channel elicits transient, fast activating, and completely desensitizing inward currents (PubMed : 21036899). Displays high selectivity for sodium ions but can also permit the permeation of other cations (PubMed : 21036899). Regulates more or less directly intracellular calcium concentration and CaMKII phosphorylation, and thereby the density of dendritic spines. Modulates neuronal activity in the circuits underlying innate fear (By similarity).. Isoform Asic1a. Has high selectivity for sodium ions, but can also be permeable to other cations including calcium, lithium and potassium.. Isoform Asic1b. Produces acid activated currents with a reduced amplitude and inactivates faster (PubMed : 21036899). Has high selectivity for sodium ions but also supports a calcium-mediated current which is sustained and maintained as long as acidic conditions are present (PubMed : 21036899). Also potentially permeable to lithium and potassium (PubMed : 21036899).. Isoform 1. Has no measurable proton-gated sodium channel activity in vitro.

Sequence similarities

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC1 subfamily.

Post-translational modifications

pH-gating could be regulated by serine proteases.. Phosphorylation by PKA regulates interaction with PICK1 and subcellular localization (PubMed:12578970). Phosphorylation by PKC may regulate the channel (PubMed:12244121).

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Product protocols

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Target data

Forms voltage-independent, pH-gated trimeric sodium channels that act as postsynaptic excitatory receptors in the nervous system, playing a crucial role in regulating synaptic plasticity, learning, and memory (PubMed : 21036899, PubMed : 32915133, PubMed : 34319232). Upon extracellular pH drop this channel elicits transient, fast activating, and completely desensitizing inward currents (PubMed : 21036899). Displays high selectivity for sodium ions but can also permit the permeation of other cations (PubMed : 21036899). Regulates more or less directly intracellular calcium concentration and CaMKII phosphorylation, and thereby the density of dendritic spines. Modulates neuronal activity in the circuits underlying innate fear (By similarity).. Isoform Asic1a. Has high selectivity for sodium ions, but can also be permeable to other cations including calcium, lithium and potassium.. Isoform Asic1b. Produces acid activated currents with a reduced amplitude and inactivates faster (PubMed : 21036899). Has high selectivity for sodium ions but also supports a calcium-mediated current which is sustained and maintained as long as acidic conditions are present (PubMed : 21036899). Also potentially permeable to lithium and potassium (PubMed : 21036899).. Isoform 1. Has no measurable proton-gated sodium channel activity in vitro.
See full target information ASIC1
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