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Recombinant Human Aspartate Aminotransferase protein is a Human protein, in the 1 to 413 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Alternative names=cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M A P P S V F A E V P Q A Q P V L V F K L T A D F R E D P D P R K V N L G V G A Y R T D D C H P W V L P V V K K V E Q K I A N D N S L N H E Y L P I L G L A E F R S C A S R L A L G D D S P A L K E K R V G G V Q S L G G T G A L R I G A D F L A R W Y N G T N N K N T P V Y V S S P T W E N H N A V F S A A G F K D I R S Y R Y W D A E K R G L D L Q G F L N D L E N A P E F S I V V L H A C A H N P T G I D P T P E Q W K Q I A S V M K H R F L F P F F D S A Y Q G F A S G N L E R D A W A I R Y F V S E G F E F F C A Q S F S K N F G L Y N E R V G N L T V V G K E P E S I L Q V L S Q M E K I V R I T W S N P P A Q G A R I V A S T L S N P E L F E E W T G N V K T M A D R I L T M R S E L R A R L E A L K T P G T W N H I T D Q I G M F S F T G L N P K Q V E Y L V N E K H I Y L L P S G R I N V S G L T T K N L D Y V A T S I H E A V T K I Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human Aspartate Aminotransferase protein is a Human protein, in the 1 to 413 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Alternative names=cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Predicted molecular weight
48.4 kDa
- Amino acids
1 to 413
- Sequence
M G S S H H H H H H S S G L V P R G S H M A P P S V F A E V P Q A Q P V L V F K L T A D F R E D P D P R K V N L G V G A Y R T D D C H P W V L P V V K K V E Q K I A N D N S L N H E Y L P I L G L A E F R S C A S R L A L G D D S P A L K E K R V G G V Q S L G G T G A L R I G A D F L A R W Y N G T N N K N T P V Y V S S P T W E N H N A V F S A A G F K D I R S Y R Y W D A E K R G L D L Q G F L N D L E N A P E F S I V V L H A C A H N P T G I D P T P E Q W K Q I A S V M K H R F L F P F F D S A Y Q G F A S G N L E R D A W A I R Y F V S E G F E F F C A Q S F S K N F G L Y N E R V G N L T V V G K E P E S I L Q V L S Q M E K I V R I T W S N P P A Q G A R I V A S T L S N P E L F E E W T G N V K T M A D R I L T M R S E L R A R L E A L K T P G T W N H I T D Q I G M F S F T G L N P K Q V E Y L V N E K H I Y L L P S G R I N V S G L T T K N L D Y V A T S I H E A V T K I Q
- Accession
- P17174-1
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
Liquid
- Additional notes
ab99147 was purified using conventional chromatography.
General info
- Function
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
- Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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