Recombinant Human Asporin protein is a Human Full Length protein, in the 1 to 384 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M K E Y V L L L F L A L C S A K P F F S P S H I A L K N M M L K D M E D T D D D D D D D D D D D D D D D D D E E D N S L F P T R E P R S H F F P F D L F P M C P F G C Q C Y S R V V H C S D L G L T S V P T N I P F D T R M L D L Q N N K I K E I K E N D F K G L T S L Y G L I L N N N K L T K I H P K A F L T T K K L R R L Y L S H N Q L S E I P L N L P K S L A E L R I H E N K V K K I Q K D T F K G M N A L H V L E M S A N P L D N N G I E P G A F E G V T V F H I R I A E A K L T S V P K G L P P T L L E L H L D Y N K I S T V E L E D F K R Y K E L Q R L G L G N N K I T D I E N G S L A N I P R V R E I H L E N N K L K K I P S G L P E L K Y L Q I I F L H S N S I A R V G V N D F C P T V P K M K K S L Y S A I S L F N N P V K Y W E M Q P A T F R C V L S R M S V Q L G N F G M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.
Alternative names
PLAP1, SLRR1C, UNQ215/PRO241, ASPN, Asporin, Periodontal ligament-associated protein 1, PLAP-1
Recommended products
Recombinant Human Asporin protein is a Human Full Length protein, in the 1 to 384 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9BXN1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M K E Y V L L L F L A L C S A K P F F S P S H I A L K N M M L K D M E D T D D D D D D D D D D D D D D D D D E E D N S L F P T R E P R S H F F P F D L F P M C P F G C Q C Y S R V V H C S D L G L T S V P T N I P F D T R M L D L Q N N K I K E I K E N D F K G L T S L Y G L I L N N N K L T K I H P K A F L T T K K L R R L Y L S H N Q L S E I P L N L P K S L A E L R I H E N K V K K I Q K D T F K G M N A L H V L E M S A N P L D N N G I E P G A F E G V T V F H I R I A E A K L T S V P K G L P P T L L E L H L D Y N K I S T V E L E D F K R Y K E L Q R L G L G N N K I T D I E N G S L A N I P R V R E I H L E N N K L K K I P S G L P E L K Y L Q I I F L H S N S I A R V G V N D F C P T V P K M K K S L Y S A I S L F N N P V K Y W E M Q P A T F R C V L S R M S V Q L G N F G M
- Accession
- Q9BXN1
- Protein length
- Full Length
- Predicted molecular weight
- 70.3 kDa
- Amino acids
- 1 to 384
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.
- Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
- Post-translational modifications
There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Asporin also known as ASPN is a small leucine-rich proteoglycan. Asporin weighs about 39 kDa. It expresses mostly in cartilage with notable presence in fibrocartilage and intervertebral discs. Scientists have identified asporin as a regulator of extracellular matrix organization and a mediator in cellular processes such as cell adhesion and migration. In Singapore and other places research on this protein has gained significant attention due to its roles in tissue development and potential clinical implications.
- Biological function summary
The protein functions in modulating the extracellular matrix and influences mineralization of tissues. It belongs to a family of small leucine-rich repeat proteins that typically form part of larger complexes. Asporin interacts with TGF-β (transforming growth factor-beta) inhibiting its signaling pathway. This interaction affects collagen fibrillogenesis and may influence tissue repair processes. The ability of asporin to bind calcium hints at its role in calcification processes as well.
- Pathways
Asporin plays a significant role in the regulation of the TGF-β and BMP (bone morphogenetic protein) signaling pathways. These pathways are key in controlling cell growth and differentiation particularly in connective tissues. Through these pathways asporin interacts with proteins like collagen I and II as well as other matrix components. It’s involved in fine-tuning processes such as skeletal development and cartilage homeostasis.
- Associated diseases and disorders
Research reveals connections between asporin and osteoarthritis as well as intervertebral disc disease. Variants in asporin known as polymorphisms have been linked to susceptibility to osteoarthritis in certain populations. Its interaction with TGF-β impacts disease progression implicating other proteins such as matrix metalloproteinases in the extracellular matrix breakdown. Asporin's modulation of tissue mineralization also relates to intervertebral disc degeneration providing insights into potential therapeutic targets.
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1 product image
SDS-PAGE - Recombinant Human Asporin protein (ab132382)
12.5% SDS-PAGE analysis of ab132382 stained with Coomassie Blue.
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