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AB187483

Recombinant Human ASRGL1 protein (His tag N-Terminus)

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Recombinant Human ASRGL1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 308 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ALP, CRASH, ASRGL1, Isoaspartyl peptidase/L-asparaginase, Asparaginase-like protein 1, Beta-aspartyl-peptidase, Isoaspartyl dipeptidase, L-asparagine amidohydrolase

1 Images
SDS-PAGE - Recombinant Human ASRGL1 protein (AB187483)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ASRGL1 protein (AB187483)

15% SDS-PAGE analysis of ab187483 (3 μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q7L266

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMNPIVVVHGGGAGPISKDRKERVHQGMVRAATVGYGILREGGSAVDAVEGAVVALEDDPEFNAGCGSVLNTNGEVEMDASIMDGKDLSAGAVSAVQCIANPIKLARLVMEKTPHCFLTDQGAAQFAAAMGVPEIPGEKLVTERNKKRLEKEKHEKGAQKTDCQKNLGTVGAVALDCKGNVAYATSTGGIVNKMVGRVGDSPCLGAGGYADNDIGAVSTTGHGESILKVNLARLTLFHIEQGKTVEEAADLSLGYMKSRVKGLGGLIVVSKTGDWVAKWTSTSMPWAAAKDGKLHFGIDPDDTTITDLP","proteinLength":"Full Length","predictedMolecularWeight":"34 kDa","actualMolecularWeight":null,"aminoAcidEnd":308,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q7L266","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASRGL1 also known as asparaginase-like protein 1 plays a role as an asparaginase enzyme. It catalyzes the hydrolysis of L-asparagine to L-aspartate and ammonia. The protein has a molecular mass of approximately 30 kDa. ASRGL1 is mainly expressed in the colon endometrium ovary and various other tissues. This expression varies indicating its involvement in multiple physiological processes across different environments within the organism.
Biological function summary

ASRGL1 contributes to the metabolism of amino acids and is involved in cellular processes that require protein catabolism. The protein is not typically part of a larger complex functioning independently to facilitate chemical reactions important for maintaining amino acid balance. Through its enzymatic activities ASRGL1 supports cellular function and protein turnover highlighting its importance in basic cellular metabolism.

Pathways

The ASRGL1 protein participates in the glutamine and glutamate metabolism pathway. It also plays a role in the urea cycle keeping a balance between nitrogen and amino acid levels. In these pathways ASRGL1 interacts with proteins like glutaminase and carbamoyl phosphate synthetase 1 which have roles in nitrogen metabolism and energy production. These interactions emphasize the protein's integration in fundamental metabolic processes.

Research shows ASRGL1's implication in ovarian cancer and neurological disorders. Abnormal expression or mutations of ASRGL1 can be linked to the progression of ovarian tumors. Moreover altered ASRGL1 activity might relate to some neurodegenerative disorders due to its role in amino acid metabolism. The protein may interact with mutated forms of other enzymes associated with these conditions such as glutamine synthetase in certain cases. Understanding these connections aids in discovering therapeutic targets for treating these diseases.

Specifications

Form

Liquid

Additional notes

ab187483 was purified using conventional chromatography.

General info

Function

Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.

Sequence similarities

Belongs to the Ntn-hydrolase family.

Post-translational modifications

Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity.

Product protocols

Target data

Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.
See full target information ASRGL1

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