Recombinant Human ASS1 protein is a Human Full Length protein, in the 1 to 412 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S S K G S V V L A Y S G G L D T S C I L V W L K E Q G Y D V I A Y L A N I G Q K E D F E E A R K K A L K L G A K K V F I E D V S R E F V E E F I W P A I Q S S A L Y E D R Y L L G T S L A R P C I A R K Q V E I A Q R E G A K Y V S H G A T G K G N D Q V R F E L S C Y S L A P Q I K V I A P W R M P E F Y N R F K G R N D L M E Y A K Q H G I P I P V T P K N P W S M D E N L M H I S Y E A G I L E N P K N Q A P P G L Y T K T Q D P A K A P N T P D I L E I E F K K G V P V K V T N V K D G T T H Q T S L E L F M Y L N E V A G K H G V G R I D I V E N R F I G M K S R G I Y E T P A G T I L Y H A H L D I E A F T M D R E V R K I K Q G L G L K F A E L V Y T G F W H S P E C E F V R H C I A K S Q E R V E G K V Q V S V L K G Q V Y I L G R E S P L S L Y N E E L V S M N V Q G D Y E P T D A T G F I N I N S L R L K E Y H R L Q S K V T A K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Alternative names
ASS, ASS1, Argininosuccinate synthase, Citrulline--aspartate ligase
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Recombinant Human ASS1 protein is a Human Full Length protein, in the 1 to 412 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P00966-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.00154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S S K G S V V L A Y S G G L D T S C I L V W L K E Q G Y D V I A Y L A N I G Q K E D F E E A R K K A L K L G A K K V F I E D V S R E F V E E F I W P A I Q S S A L Y E D R Y L L G T S L A R P C I A R K Q V E I A Q R E G A K Y V S H G A T G K G N D Q V R F E L S C Y S L A P Q I K V I A P W R M P E F Y N R F K G R N D L M E Y A K Q H G I P I P V T P K N P W S M D E N L M H I S Y E A G I L E N P K N Q A P P G L Y T K T Q D P A K A P N T P D I L E I E F K K G V P V K V T N V K D G T T H Q T S L E L F M Y L N E V A G K H G V G R I D I V E N R F I G M K S R G I Y E T P A G T I L Y H A H L D I E A F T M D R E V R K I K Q G L G L K F A E L V Y T G F W H S P E C E F V R H C I A K S Q E R V E G K V Q V S V L K G Q V Y I L G R E S P L S L Y N E E L V S M N V Q G D Y E P T D A T G F I N I N S L R L K E Y H R L Q S K V T A K
- Accession
- P00966
- Protein length
- Full Length
- Predicted molecular weight
- 48.6 kDa
- Amino acids
- 1 to 412
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab98084 was purified using conventional chromatography techniques.
General info
- Function
One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
- Sequence similarities
Belongs to the argininosuccinate synthase family. Type 1 subfamily.
- Post-translational modifications
Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.
- Biological function summary
Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.
- Pathways
ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.
- Associated diseases and disorders
ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.
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1 product image
SDS-PAGE - Recombinant Human ASS1 protein (ab98084)
15% SDS-PAGE analysis of ab98084 (3μg)
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