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AB127446

Recombinant Human ATBF1 protein

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Recombinant Human ATBF1 protein is a Human Fragment protein, in the 517 to 787 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE.

View Alternative Names

ATBF1, C16orf47, ZFHX3, Zinc finger homeobox protein 3, AT motif-binding factor 1, AT-binding transcription factor 1, Alpha-fetoprotein enhancer-binding protein, Zinc finger homeodomain protein 3, ZFH-3

Key facts

Purity

>70% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q15911

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"29 kDa","actualMolecularWeight":null,"aminoAcidEnd":787,"aminoAcidStart":517,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15911","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATBF1 also known as AT motif-binding factor 1 is a transcription factor that modulates gene expression. It is a large protein with a mass of approximately 404 kDa. You can find its expression in numerous tissues including the brain liver and prostate. ATBF1 contains several zinc finger domains that allow it to bind DNA and regulate transcription of target genes influencing cellular proliferation and differentiation.
Biological function summary

The function of AT motif-binding factor 1 extends to various cellular processes through its role as a transcriptional regulator. ATBF1 typically forms part of a larger complex in the nucleus interacting with other transcription factors to modulate gene expression. ATBF1 represses or activates transcription depending on the cellular context and target genes playing an important role in growth and development. For example it negatively regulates the expression of genes involved in cell cycle progression thereby acting as a suppressor in certain contexts.

Pathways

The molecular activities of AT motif-binding factor 1 are pivotal in the regulation of the Wnt and TGF-beta signaling pathways. These pathways are critical for cell proliferation differentiation and apoptosis. In the Wnt pathway ATBF1 interacts with beta-catenin modulating transcription of specific target genes that impact cell fate. In the TGF-beta pathway it affects various downstream signaling molecules influencing cellular responses to growth factor stimuli and maintaining cellular homeostasis.

You will often see AT motif-binding factor 1 associated with prostate cancer and liver disease. In prostate cancer reduced expression or mutations in ATBF1 have been linked to tumor progression often working in conjunction with the protein AR (androgen receptor). In liver disease alterations in ATBF1 function can disrupt normal liver cell differentiation and proliferation associating it with hepatocellular carcinoma. Understanding these interactions offers insights into potential therapeutics targeting ATBF1 in these conditions.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Transcriptional regulator which can act as an activator or a repressor. Inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. In concert with SMAD-dependent TGF-beta signaling can repress the transcription of AFP via its interaction with SMAD2/3 (PubMed : 25105025). Regulates the circadian locomotor rhythms via transcriptional activation of neuropeptidergic genes which are essential for intercellular synchrony and rhythm amplitude in the suprachiasmatic nucleus (SCN) of the brain (By similarity). Regulator of myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression (PubMed : 11312261). Down-regulates the MUC5AC promoter in gastric cancer (PubMed : 17330845). In association with RUNX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed : 20599712). Inhibits estrogen receptor (ESR1) function by selectively competing with coactivator NCOA3 for binding to ESR1 in ESR1-positive breast cancer cells (PubMed : 20720010).

Post-translational modifications

Hyperphosphorylation protects ZFHX3 from calpain/CAPN1-mediated degradation.. Ubiquitinated, leading to its proteasomal degradation.. Nuclear localization is essential for its sumoylation.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator which can act as an activator or a repressor. Inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. In concert with SMAD-dependent TGF-beta signaling can repress the transcription of AFP via its interaction with SMAD2/3 (PubMed : 25105025). Regulates the circadian locomotor rhythms via transcriptional activation of neuropeptidergic genes which are essential for intercellular synchrony and rhythm amplitude in the suprachiasmatic nucleus (SCN) of the brain (By similarity). Regulator of myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression (PubMed : 11312261). Down-regulates the MUC5AC promoter in gastric cancer (PubMed : 17330845). In association with RUNX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed : 20599712). Inhibits estrogen receptor (ESR1) function by selectively competing with coactivator NCOA3 for binding to ESR1 in ESR1-positive breast cancer cells (PubMed : 20720010).
See full target information ZFHX3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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