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AB225977

Recombinant Human ATG14L protein (Tagged)

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Recombinant Human ATG14L protein (Tagged) is a Human Full Length protein, in the 1 to 492 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

ATG14L, KIAA0831, ATG14, Beclin 1-associated autophagy-related key regulator, Barkor, Autophagy-related protein 14-like protein, Atg14L

1 Images
SDS-PAGE - Recombinant Human ATG14L protein (Tagged) (AB225977)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ATG14L protein (Tagged) (AB225977)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225977 with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q6ZNE5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR","proteinLength":"Full Length","predictedMolecularWeight":"60.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":492,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6ZNE5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG14L also known as BARKOR is an essential protein involved in the formation of autophagosomes organelles which sequester cytoplasmic components for degradation. This protein is approximately 55 kDa in mass. ATG14L is expressed in a variety of tissues including high expression in the brain heart and kidney. It plays an important role in mediating the recruitment of the PI3K (phosphatidylinositol 3-kinase) complex to the isolation membrane a step necessary in autophagy initiation.
Biological function summary

ATG14L coordinates with other proteins as part of the PI3K complex performing a central function in the regulation of autophagy the cellular degradation process critical for maintaining cellular homeostasis. This complex includes VPS34 VPS15 and Beclin 1 which work together to facilitate the nucleation of the autophagosome membrane. Autophagy driven by ATG14L is important for cellular responses to nutrient deprivation and stress.

Pathways

ATG14L is important for two central pathways: the regulation of autophagy and the PI3K/AKT/mTOR signaling pathways. ATG14L directly affects the activity of mTORC1 an important regulator of cell growth and metabolism integrating signals from nutrients and growth factors. It interacts with other autophagy-related proteins such as Beclin 1 to modulate these pathways connecting intracellular energy status with metabolic processes.

ATG14L has a significant connection to neurodegenerative diseases and cancer. Altered autophagy with ATG14L malfunction contributes to the progression of neurodegenerative diseases like Alzheimer's disease where abnormal protein aggregates are not efficiently degraded. In the context of cancer dysregulation of ATG14L-related autophagy pathways can either promote or inhibit tumor growth making it an important therapeutic target. It shares complex interactions with Beclin 1 in these disorders further emphasizing its importance in disease mechanisms.
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Specifications

Form

Liquid

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General info

Function

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed : 18843052, PubMed : 19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed : 19270696, PubMed : 20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed : 20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed : 23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed : 23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed : 25686604, PubMed : 37632749). Modulates the hepatic lipid metabolism (By similarity).

Sequence similarities

Belongs to the ATG14 family.

Post-translational modifications

Ubiquitinated via 'Lys-6', 'Lys-11' and 'Lys-63'-linked polyubiquitin chains on multiple lysines by MARCHF7, leading to ATG14 aggregation and loss of interaction with STX17.

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Product protocols

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Target data

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed : 18843052, PubMed : 19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed : 19270696, PubMed : 20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed : 20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed : 23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed : 23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed : 25686604, PubMed : 37632749). Modulates the hepatic lipid metabolism (By similarity).
See full target information ATG14
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