Recombinant human ATP1B1 protein (Active) is a Human Fragment protein, in the 63 to 303 aa range, expressed in Baculovirus infected Sf21, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected Sf21 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A D P E F K P T Y Q D R V A P P G L T Q I P Q I Q K T E I S F R P N D P K S Y E A Y V L N I V R F L E K Y K D S A Q R D D M I F E D C G D V P S E P K E R G D F N H E R G E R K V C R F K L E W L G N C S G L N D E T Y G Y K E G K P C I I I K L N R V L G F K P K P P K N E S L E T Y P V M K Y N P N V L P V Q C T G K R D E D K D K V G N V E Y F G L G N S P G F P L Q Y Y P Y Y G K L L Q P K Y L Q P L L A V Q F T N L T M D T E I R I E C K A Y G E N I G Y S E K D R F Q G R F D V K I E V K S H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane (PubMed:19694409). Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1 (PubMed:34011520). Involved in cell adhesion and establishing epithelial cell polarity.
Alternative names
ATP1B, ATP1B1, Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1
Recommended products
Recombinant human ATP1B1 protein (Active) is a Human Fragment protein, in the 63 to 303 aa range, expressed in Baculovirus infected Sf21, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected Sf21 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 3,000 pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25°C.
- Accession
- P05026-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P E F K P T Y Q D R V A P P G L T Q I P Q I Q K T E I S F R P N D P K S Y E A Y V L N I V R F L E K Y K D S A Q R D D M I F E D C G D V P S E P K E R G D F N H E R G E R K V C R F K L E W L G N C S G L N D E T Y G Y K E G K P C I I I K L N R V L G F K P K P P K N E S L E T Y P V M K Y N P N V L P V Q C T G K R D E D K D K V G N V E Y F G L G N S P G F P L Q Y Y P Y Y G K L L Q P K Y L Q P L L A V Q F T N L T M D T E I R I E C K A Y G E N I G Y S E K D R F Q G R F D V K I E V K S H H H H H H
- Accession
- P05026
- Protein length
- Fragment
- Predicted molecular weight
- 29 kDa
- Amino acids
- 63 to 303
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane (PubMed:19694409). Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1 (PubMed:34011520).
- Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family.
- Post-translational modifications
Glutathionylated (By similarity). N-glycosylated (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Shi et al. Overexpression of ATP1B1 predicts an adverse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget. 2016 Jan 19;7(3):2585-95. doi: 10.18632/oncotarget.6226.
This product was previously labelled as beta 1 Sodium Potassium ATPase
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP1B1 also known as the beta-1 subunit of Na+/K+ ATPase plays an essential role in ion transport across the plasma membrane. The protein works as part of the sodium-potassium pump which helps maintain the cellular electrochemical gradient. The molecular mass of ATP1B1 is approximately 35 kDa. This target is expressed in various tissues including the heart kidney and brain where it supports the critical functions of excitable tissues and epithelial cell polarization.
- Biological function summary
ATP1B1 contributes to establishing and maintaining cellular homeostasis as part of the Na+/K+ ATPase complex. This complex regulates the balance of sodium and potassium ions within cells important for nerve impulse transmission and muscle contraction. ATP1B1 interacts with the alpha subunit to modulate the enzyme’s activity and transport kinetics highlighting its role in cell volume regulation and epithelial layer development.
- Pathways
ATP1B1 is actively involved in the ion transport pathway and cellular signaling pathways such as WNT signaling. It works closely with proteins such as ATP1A1 and ATP1A2 which are different alpha subunits of the Na+/K+ ATPase. Through these pathways ATP1B1 affects cellular processes like membrane potential maintenance and intercellular communication essential for proper cellular function and organismal health.
- Associated diseases and disorders
ATP1B1 shows a link to hypertension and polycystic ovary syndrome (PCOS). Mutations or dysfunctional expression of ATP1B1 can disrupt ion gradients contributing to these conditions. The protein connects with regulatory proteins including G-protein signaling regulators which can alter cellular responses in disease contexts. Understanding ATP1B1's involvement in these disorders helps in exploring therapeutic targets and improving patient outcomes.
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1 product image
SDS-PAGE - Recombinant human ATP1B1 protein (Active) (ab222961)
SDS-PAGE - Recombinant human ATP1B1 protein (Active) (3 μg ab222961).
28-40 KDa under reducing conditions.
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