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AB157947

Recombinant Human ATP6AP1/ATP6S1 protein

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Recombinant Human ATP6AP1/ATP6S1 protein is a Human Full Length protein, in the 1 to 470 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ATP6IP1, ATP6S1, VATPS1, XAP3, ATP6AP1, V-type proton ATPase subunit S1, V-ATPase subunit S1, Protein XAP-3, V-ATPase Ac45 subunit, V-ATPase S1 accessory protein, Vacuolar proton pump subunit S1

1 Images
SDS-PAGE - Recombinant Human ATP6AP1/ATP6S1 protein (AB157947)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ATP6AP1/ATP6S1 protein (AB157947)

ab157947 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q15904

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as ATP6AP1.
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Sequence info

[{"sequence":"MMAAMATARVRMGPRCAQALWRMPWLPVFLSLAAAAAAAAAEQQVPLVLWSSDRDLWAPAADTHEGHITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYTASSGLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPSRVARDVAVVAGGLGRQLLQKQPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWNDSFARLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGPSIYSFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFFSPGIWMGLLTSLFMLFIFTYGLHMILSLKTMDRFDDHKGPTISLTQIV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":470,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q15904","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6AP1 also known as ATPase H+ Transporting Accessory Protein 1 is a component of the V-ATPase complex. This protein weighing about 55 kDa assists in the acidification of organelles such as endosomes and lysosomes. ATP6AP1 exists mainly in the endoplasmic reticulum and is also found in the Golgi apparatus. It interacts with other proteins to maintain proper pH levels in intracellular compartments which is important for various cellular processes.
Biological function summary

ATP6AP1 acts as a regulatory subunit of the vacuolar ATPase (V-ATPase) proton pump complex. This complex is responsible for pumping protons across intracellular and plasma membranes which acidifies vacuoles and lysosomal compartments. Such acidification is necessary for degradation processes within lysosomes. Its association with V-ATPase shows that ATP6AP1 influences pH balance rather than enzyme activity playing a role in cellular homeostasis and trafficking.

Pathways

ATP6AP1 functions within both the acidification and autophagy pathways. Maintaining the acidic environment needed for endocytic and autophagic pathways ensures proper degradation and recycling of cellular components. ATP6AP1 associates with proteins like ATP6V1A an important component of the V-ATPase linking it to proton translocation processes. The protein’s activity within these pathways supports vesicle acidification needed for processes like protein degradation and cellular waste elimination.

ATP6AP1 mutations have connections to glycosylation disorders and congenital disorders of glycosylation type IIH. Alterations in the gene encoding ATP6AP1 can lead to improper protein glycosylation and trafficking within the cell. This malfunction is associated with symptoms that affect multiple systems and cause developmental issues. Additionally ATP6AP1 has an established relationship with another protein ATP6V0A2 through pathways linked to glycosylation demonstrating its importance in maintaining cellular function and health.
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Specifications

Form

Liquid

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General info

Function

Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles (PubMed : 33065002). Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity (PubMed : 27231034). Involved in membrane trafficking and Ca(2+)-dependent membrane fusion (PubMed : 27231034). May play a role in the assembly of the V-type ATPase complex (Probable). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed : 28296633). In islets of Langerhans cells, may regulate the acidification of dense-core secretory granules (By similarity).

Sequence similarities

Belongs to the vacuolar ATPase subunit S1 family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles (PubMed : 33065002). Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity (PubMed : 27231034). Involved in membrane trafficking and Ca(2+)-dependent membrane fusion (PubMed : 27231034). May play a role in the assembly of the V-type ATPase complex (Probable). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed : 28296633). In islets of Langerhans cells, may regulate the acidification of dense-core secretory granules (By similarity).
See full target information ATP6AP1
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