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AB157939

Recombinant Human ATP6V1B1 protein (GST tag N-Terminus)

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Recombinant Human ATP6V1B1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 513 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ATP6B1, VATB, VPP3, ATP6V1B1, V-ATPase subunit B 1, Endomembrane proton pump 58 kDa subunit, Vacuolar proton pump subunit B 1

1 Images
SDS-PAGE - Recombinant Human ATP6V1B1 protein (GST tag N-Terminus) (AB157939)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ATP6V1B1 protein (GST tag N-Terminus) (AB157939)

ab157939 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P15313

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB200839

Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB200839)

  • 0
  • 0
Primary Antibodies

AB73404

Anti-ATP6V1B2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B2 antibody (AB73404)

  • 5
  • 1
Primary Antibodies

AB187706

Anti-ATP6V1H antibody

Immunoprecipitation - Anti-ATP6V1H antibody (AB187706)

  • 0
  • 0
Primary Antibodies

AB97440

Anti-ATP6V0A4 antibody

Western blot - Anti-ATP6V0A4 antibody (AB97440)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAMEIDSRPGGLPGSSCNLGAAREHMQAVTRNYITHPRVTYRTVCSVNGPLVVLDRVKFAQYAEIVHFTLPDGTQRSGQVLEVAGTKAIVQVFEGTSGIDARKTTCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPVVMAEDFLDINGQPINPHSRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKAVLDYHDDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRGGSITQIPILTMPNDDITHPIPDLTGFITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYACYAIGKDVQAMKAVVGEEALTSEDLLYLEFLQKFEKNFINQGPYENRSVFESLDLGWKLLRIFPKEMLKRIPQAVIDEFYSREGALQDLAPDTAL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":513,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P15313","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1B1 also known as V-type proton ATPase subunit B1 is an important component of the vacuolar ATPase (V-ATPase) complex. It plays an important role in the acidification of intracellular compartments by facilitating proton translocation across membranes. The molecular mass of ATP6V1B1 is approximately 58 kDa. ATP6V1B1 expresses prominently in the kidney specifically in the epithelial cells of the distal tubules and collecting ducts as well as in the inner ear.
Biological function summary

ATP6V1B1 is involved in cellular processes that require the maintenance of pH gradients across membranes. It forms part of the V-ATPase complex which consists of multiple subunits working together to pump protons into the vacuoles and other organelles effectuating an acidic environment. These acidic environments are important for protein sorting zymogen activation and receptor-mediated endocytosis.

Pathways

ATP6V1B1 contributes directly to the endocytic and lysosomal degradation pathways playing an essential role in cellular homeostasis. The protein interacts with other V-ATPase subunits such as ATP6V1E1 and ATP6V1C1 within these pathways to ensure the proper functioning of organelles. It also assists in maintaining the acidification essential for the processing and transport of molecules inside cells.

Mutations in the ATP6V1B1 gene associate strongly with distal renal tubular acidosis (dRTA) and sensorineural hearing loss. In dRTA disrupted acidification in the kidney impairs the body's ability to excrete acids or balance pH. Furthermore ATP6V1B1's interaction with proteins like ATP6V0A4 is significant as mutations in both proteins are linked to a similar disorder highlighting their role in maintaining renal and auditory function.
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Specifications

Form

Liquid

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General info

Function

Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 16769747). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed : 32001091). Essential for the proper assembly and activity of V-ATPase (PubMed : 16769747). In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed : 16769747). Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (By similarity).

Sequence similarities

Belongs to the ATPase alpha/beta chains family.

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Product protocols

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Target data

Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 16769747). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed : 32001091). Essential for the proper assembly and activity of V-ATPase (PubMed : 16769747). In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed : 16769747). Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (By similarity).
See full target information ATP6V1B1
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