Recombinant human Axl protein is a Human Fragment protein, in the 473 to 894 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
H R R K K E T R Y G E V F E P T V E R G E L V V R Y C V R K S Y S R R T T E A T L N S L G I S E E L K E K L R D V M V D R H K V A L G K T L G E G E F G A V M E G Q L N Q D D S I L K V A V K T M K I A I C T R S E L E D F L S E A V C M K E F D H P N V M R L I G V C F Q G S E R E S F P A P V V I L P F M K H G D L H S F L L Y S R L G D Q P V Y L P T Q M L V K F M A D I A S G M E Y L S T K R F I H R D L A A R N C M L N E N M S V C V A D F G L S K K I Y N G D Y Y R Q G R I A K M P V K W I A I E S L A D R V Y T S K S D V W S F G V T M W E I A T R G Q T P Y P G V E N S E I Y D Y L R Q G N R L K Q P A D C L D G L Y A L M S R C W E L N P Q D R P S F T E L R E D L E N T L K A L P P A Q E P D E I L Y V N M D E G G G Y P E P P G A A G G A D P P T Q P D P K D S C S C L T A A E V H P A G R Y V L C P S T T P S P A Q P A D R G S P A A P G Q E D G A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. (Microbial infection) Promotes Zika virus entry in glial cells, Sertoli cells and astrocytes (PubMed:28076778, PubMed:29379210, PubMed:31311882). Additionally, Zika virus potentiates AXL kinase activity to antagonize type I interferon signaling and thereby promotes infection (PubMed:28076778). Interferon signaling inhibition occurs via an SOCS1-dependent mechanism (PubMed:29379210).
Alternative names
UFO, AXL, Tyrosine-protein kinase receptor UFO, AXL oncogene
Recommended products
Recombinant human Axl protein is a Human Fragment protein, in the 473 to 894 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab167950 was determined to be 3 nmol/min/mg.
- Accession
- P30530-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- H R R K K E T R Y G E V F E P T V E R G E L V V R Y C V R K S Y S R R T T E A T L N S L G I S E E L K E K L R D V M V D R H K V A L G K T L G E G E F G A V M E G Q L N Q D D S I L K V A V K T M K I A I C T R S E L E D F L S E A V C M K E F D H P N V M R L I G V C F Q G S E R E S F P A P V V I L P F M K H G D L H S F L L Y S R L G D Q P V Y L P T Q M L V K F M A D I A S G M E Y L S T K R F I H R D L A A R N C M L N E N M S V C V A D F G L S K K I Y N G D Y Y R Q G R I A K M P V K W I A I E S L A D R V Y T S K S D V W S F G V T M W E I A T R G Q T P Y P G V E N S E I Y D Y L R Q G N R L K Q P A D C L D G L Y A L M S R C W E L N P Q D R P S F T E L R E D L E N T L K A L P P A Q E P D E I L Y V N M D E G G G Y P E P P G A A G G A D P P T Q P D P K D S C S C L T A A E V H P A G R Y V L C P S T T P S P A Q P A D R G S P A A P G Q E D G A
- Accession
- P30530
- Protein length
- Fragment
- Predicted molecular weight
- 76 kDa
- Amino acids
- 473 to 894
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
- Post-translational modifications
Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Axl protein also known as AXL receptor tyrosine kinase plays a significant role in cell signaling. It has a molecular weight of approximately 98 kDa. Axl is a transmembrane receptor that is expressed in a variety of tissues including the immune system reproductive organs and the central nervous system. It is mainly recognized for transmitting signals from the extracellular matrix into the cytoplasm by binding with its ligand Gas6.
- Biological function summary
Axl is important in mediating cell survival proliferation and migration. It functions as part of the TAM family of receptors which also includes Tyro3 and Mer. Axl often forms complexes with these receptors to facilitate efficient signaling. This interaction triggers phosphorylation events that activate downstream signaling proteins reinforcing its influence in cellular functions.
- Pathways
Axl is deeply involved in the PI3K-Akt and MAPK signaling pathways which are central to cellular growth and survival. In these pathways Axl interacts closely with proteins like PI3K and Akt to regulate processes such as apoptosis and metabolism. Its interaction with these pathways places Axl as an important modulator of intracellular signaling cascades.
- Associated diseases and disorders
Axl has been implicated in cancer progression and immune response dysregulation. In many cancer types Axl overexpression links to tumor invasion metastasis and resistance to therapy. Moreover Axl’s role in autoimmune diseases becomes evident through its association with the Gas6 protein. Exploring these connections furthers understanding of how Axl's aberrant activity influences health and disease.
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4 product images
Functional Studies - Recombinant human Axl protein (ab167950)
The specific activity of Axl (ab167950) was determined to be 3.5 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human Axl protein (ab167950)
SDS PAGE analysis of ab167950
Functional Studies - Recombinant human Axl protein (ab167950)
The specific activity of ab167950 was determined to be 3 nmol/min/mg.
SDS-PAGE - Recombinant human Axl protein (ab167950)
SDS Page analysis of ab167950
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