Recombinant Human B3GALTL protein (His tag) is a Human Fragment protein, in the 29 to 494 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S E D T K K E V K Q S Q D L E K S G I S R K N D I D L K G I V F V I Q S Q S N S F H A K R A E Q L K K S I L K Q A A D L T Q E L P S V L L L H Q L A K Q E G A W T I L P L L P H F S V T Y S R N S S W I F F C E E E T R I Q I P K L L E T L R R Y D P S K E W F L G K A L H D E E A T I I H H Y A F S E N P T V F K Y P D F A A G W A L S I P L V N K L T K R L K S E S L K S D F T I D L K H E I A L Y I W D K G G G P P L T P V P E F C T N D V D F Y C A T T F H S F L P L C R K P V K K K D I F V A V K T C K K F H G D R I P I V K Q T W E S Q A S L I E Y Y S D Y T E N S I P T V D L G I P N T D R G H C G K T F A I L E R F L N R S Q D K T A W L V I V D D D T L I S I S R L Q H L L S C Y D S G E P V F L G E R Y G Y G L G T G G Y S Y I T G G G G M V F S R E A V R R L L A S K C R C Y S N D A P D D M V L G M C F S G L G I P V T H S P L F H Q A R P V D Y P K D Y L S H Q V P I S F H K H W N I D P V K V Y F T W L A P S D E D K A R Q E T Q K G F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
O-glucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. Specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.
Alternative names
B3GALTL, B3GTL, B3GLCT, Beta3Glc-T, Beta 3-glucosyltransferase, Beta-3-glycosyltransferase-like
Recommended products
Recombinant Human B3GALTL protein (His tag) is a Human Fragment protein, in the 29 to 494 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q6Y288-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- S E D T K K E V K Q S Q D L E K S G I S R K N D I D L K G I V F V I Q S Q S N S F H A K R A E Q L K K S I L K Q A A D L T Q E L P S V L L L H Q L A K Q E G A W T I L P L L P H F S V T Y S R N S S W I F F C E E E T R I Q I P K L L E T L R R Y D P S K E W F L G K A L H D E E A T I I H H Y A F S E N P T V F K Y P D F A A G W A L S I P L V N K L T K R L K S E S L K S D F T I D L K H E I A L Y I W D K G G G P P L T P V P E F C T N D V D F Y C A T T F H S F L P L C R K P V K K K D I F V A V K T C K K F H G D R I P I V K Q T W E S Q A S L I E Y Y S D Y T E N S I P T V D L G I P N T D R G H C G K T F A I L E R F L N R S Q D K T A W L V I V D D D T L I S I S R L Q H L L S C Y D S G E P V F L G E R Y G Y G L G T G G Y S Y I T G G G G M V F S R E A V R R L L A S K C R C Y S N D A P D D M V L G M C F S G L G I P V T H S P L F H Q A R P V D Y P K D Y L S H Q V P I S F H K H W N I D P V K V Y F T W L A P S D E D K A R Q E T Q K G F
- Accession
- Q6Y288
- Protein length
- Fragment
- Predicted molecular weight
- 55.4 kDa
- Amino acids
- 29 to 494
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
O-glucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. Specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.
- Sequence similarities
Belongs to the glycosyltransferase 31 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The B3GALTL protein also known as beta-13-galactosyltransferase-like protein is an enzyme with an approximate mass of 65 kDa. It adds galactose to specific glycoproteins and plays a role in the O-fucosylation of thrombospondin type 1 repeats. B3GALTL is expressed in various tissues including the placenta and brain. It participates in glycosylation processes that are essential for protein function and stability within the cell.
- Biological function summary
The B3GALTL protein participates in glycosylation mechanisms necessary for the functionality of numerous proteins. This enzyme acts independently rather than as part of a complex to catalyze glycosidic bond formation which is integral to modifying proteins like Notch and Thrombospondin. It ensures that proteins acquire correct structural conformations optimizing their interactions and functions within the cellular environment.
- Pathways
B3GALTL engages in the Notch signaling pathway and the biosynthesis of glycan structures. This protein's glycosylation activity directly impacts these pathways influencing cellular communication and signaling. It interacts with proteins like fucosyltransferases within these processes further highlighting its role in modifying the activity and stability of signaling pathways vital for cellular functions.
- Associated diseases and disorders
B3GALTL mutations associate with Peters Plus syndrome a genetic condition characterized by developmental delays and eye abnormalities. Another disorder linked to this protein is Atrial Septal Defect associated with thrombosis abnormalities. In these contexts B3GALTL interacts with proteins involved in vascularization and ocular development emphasizing its importance in maintaining cellular and tissue homeostasis.
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1 product image
SDS-PAGE - Recombinant Human B3GALTL protein (His tag) (ab276604)
SDS-PAGE analysis of ab276604
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