Recombinant Human B3GAT1 protein (Fc Chimera) is a Human Fragment protein, in the 28 to 334 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
T L A P L L A V H K D E G S D P R R E T P P G A D P R E Y C T S D R D I V E V V R T E Y V Y T R P P P W S D T L P T I H V V T P T Y S R P V Q K A E L T R M A N T L L H V P N L H W L V V E D A P R R T P L T A R L L R D T G L N Y T H L H V E T P R N Y K L R G D A R D P R I P R G T M Q R N L A L R W L R E T F P R N S S Q P G V V Y F A D D D N T Y S L E L F E E M R S T R R V S V W P V A F V G G L R Y E A P R V N G A G K V V G W K T V F D P H R P F A I D M A G F A V N L R L I L Q R S Q A Y F K L R G V K G G Y Q E S S L L R E L V T L N D L E P K A A N C T K I L V W H T R T E K P V L V N E G K K G F T D P S V E I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
Alternative names
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, GlcAT-P, GlcUAT-P, B3GAT1, GLCATP
Recommended products
Recombinant Human B3GAT1 protein (Fc Chimera) is a Human Fragment protein, in the 28 to 334 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q9P2W7-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
T L A P L L A V H K D E G S D P R R E T P P G A D P R E Y C T S D R D I V E V V R T E Y V Y T R P P P W S D T L P T I H V V T P T Y S R P V Q K A E L T R M A N T L L H V P N L H W L V V E D A P R R T P L T A R L L R D T G L N Y T H L H V E T P R N Y K L R G D A R D P R I P R G T M Q R N L A L R W L R E T F P R N S S Q P G V V Y F A D D D N T Y S L E L F E E M R S T R R V S V W P V A F V G G L R Y E A P R V N G A G K V V G W K T V F D P H R P F A I D M A G F A V N L R L I L Q R S Q A Y F K L R G V K G G Y Q E S S L L R E L V T L N D L E P K A A N C T K I L V W H T R T E K P V L V N E G K K G F T D P S V E I
- Accession
- Protein length
Fragment
- Predicted molecular weight
61.7 kDa
- Amino acids
28 to 334
- Nature
Recombinant
- Tags
Fc tag N-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
- Sequence similarities
Belongs to the glycosyltransferase 43 family.
- Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The B3GAT1 protein also known as CD57 or HNK-1 functions mechanically as a β-13-glucuronyltransferase. It has a mass of approximately 55 kDa. B3GAT1 localizes mainly in the Golgi apparatus and is predominantly expressed in neural and immune tissues. The protein plays an important role in the biosynthesis of the HNK-1 carbohydrate epitope which is significant for cellular communication and molecular interactions in the nervous system.
- Biological function summary
B3GAT1 participates in the generation of specific carbohydrate structures that contribute to cell-cell adhesion processes. It is a part of a larger complex of glycosyltransferases that include enzymes like β3GalT1 and ST3GalT6 which together help build complex glycan chains. By modifying glycoproteins and glycolipids B3GAT1 influences neural connectivity and immune response modulation.
- Pathways
B3GAT1 influences more than four words with its role in the glycosphingolipid biosynthesis pathway and the neural crest cell migration pathway. In glycosphingolipid biosynthesis its activity is essential for creating carbohydrate polymer structures linking it with proteins like β3GalT2. It also interacts with NCAM1 within the neural crest cell migration pathway impacting neural development and plasticity.
- Associated diseases and disorders
Several words need to be mentioned regarding B3GAT1's involvement in neurological disorders and certain types of cancers. Abnormal expression or mutations in B3GAT1 link to diseases such as Alzheimer's as the HNK-1 epitope expression correlates with neuronal impairment. In cancers altered B3GAT1 glycosylation patterns associate with tumor progression and immune escape mechanisms where its interaction with CD56 plays a role in modulating immune responses.
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1 product image
SDS-PAGE - Recombinant Human B3GAT1 protein (Fc Chimera) (ab276732)
SDS-PAGE analysis of ab276732
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