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AB276629

Recombinant Human B3GNT protein (His tag)

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Recombinant Human B3GNT protein (His tag) is a Human Fragment protein, in the 43 to 415 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

B3GNT1, B3GNT6, B4GAT1, Poly-N-acetyllactosamine extension enzyme, iGnT

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SDS-PAGE - Recombinant Human B3GNT protein (His tag) (AB276629)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human B3GNT protein (His tag) (AB276629)

SDS-PAGE analysis of ab276629

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O43505

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 100% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"DQYFEFFPPSPRSVDQVKAQLRTALASGGVLDASGDYRVYRGLLKTTMDPNDVILATHASVDNLLHLSGLLERWEGPLSVSVFAATKEEAQLATVLAYALSSHCPDMRARVAMHLVCPSRYEAAVPDPREPGEFALLRSCQEVFDKLARVAQPGINYALGTNVSYPNNLLRNLAREGANYALVIDVDMVPSEGLWRGLREMLDQSNQWGGTALVVPAFEIRRARRMPMNKNELVQLYQVGEVRPFYYGLCTPCQAPTNYSRWVNLPEESLLRPAYVVPWQDPWEPFYVAGGKVPTFDERFRQYGFNRISQACELHVAGFDFEVLNEGFLVHKGFKEALKFHPQKEAENQHNKILYRQFKQELKAKYPNSPRRC","proteinLength":"Fragment","predictedMolecularWeight":"44.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":415,"aminoAcidStart":43,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"O43505","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B3GNT also known as Beta-13-N-acetylglucosaminyltransferase is an enzyme that facilitates the transfer of N-acetylglucosamine from UDP-GlcNAc to various substrates. It often involves the synthesis of poly-N-acetyllactosamine chains. B3GNT family proteins weigh around 45 kDa although variations might exist. This enzyme mostly expresses in tissues such as the liver spleen and lungs. During mechanical processes B3GNT operates in the Golgi apparatus and actively participates in glycosylation processes.
Biological function summary

B3GNT enzymes play an important role in creating and modifying glycan structures on glycoproteins and glycolipids. Complex assemblies form with other glycosyltransferases for the efficient construction and regulation of glycan chains. These glycan structures are important for cellular processes like cell adhesion migration and signal transduction. By shaping these vital cellular events B3GNT contributes to fundamental biological functions and cell interactions.

Pathways

B3GNT performs essential functions in the glycosphingolipid biosynthesis and Notch signaling pathways. These pathways govern cell fate determination morphogenesis and differentiation. B3GNT interacts with various proteins including fucosyltransferases and sialyltransferases during the synthesis of complex glycan structures. The Notch signaling pathway also involves proteins such as Notch receptors and ligands where B3GNT influences receptor-ligand interactions through glycosylation modifications impacting the signaling outcomes.

B3GNT dysfunction correlates with conditions like cancer and congenital disorders of glycosylation. Changes in glycan structures due to altered B3GNT activity often link with tumorigenesis and cancer metastasis. B3GNT's relationship with proteins like E-cadherin in cancer can affect E-cadherin-mediated cell adhesion promoting invasive behavior. In congenital disorders of glycosylation irregularities in glycan structures reflect defects in early developmental processes leading to multisystemic symptoms. These conditions emphasize the importance of B3GNT in maintaining normal cellular functions.
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Specifications

Form

Lyophilized

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General info

Function

Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1) (PubMed : 19587235, PubMed : 23359570, PubMed : 25279697, PubMed : 25279699). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE1, during synthesis of phosphorylated O-mannosyl glycan (PubMed : 25279697, PubMed : 25279699). Phosphorylated O-mannosyl glycan is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed : 25279697, PubMed : 25279699). Required for axon guidance; via its function in O-mannosylation of alpha-dystroglycan (DAG1) (By similarity).

Sequence similarities

Belongs to the glycosyltransferase 49 family.

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Product protocols

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Target data

Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1) (PubMed : 19587235, PubMed : 23359570, PubMed : 25279697, PubMed : 25279699). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE1, during synthesis of phosphorylated O-mannosyl glycan (PubMed : 25279697, PubMed : 25279699). Phosphorylated O-mannosyl glycan is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed : 25279697, PubMed : 25279699). Required for axon guidance; via its function in O-mannosylation of alpha-dystroglycan (DAG1) (By similarity).
See full target information B4GAT1
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