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AB191634

Recombinant human B7H4 protein

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Recombinant human B7H4 protein is a Human Fragment protein, in the 29 to 258 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

B7H4, UNQ659/PRO1291, VTCN1, V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4, B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7-H4

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Functional Studies - Recombinant human B7H4 protein (AB191634)
  • FuncS

Unknown

Functional Studies - Recombinant human B7H4 protein (AB191634)

Immobilized Human B7-H4, His Tag at 2 µg/mL can bind Anti-B7-H4 MAb (Human IgG1) with a linear range of 0.4-6 ng/mL.

SDS-PAGE - Recombinant human B7H4 protein (AB191634)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human B7H4 protein (AB191634)

SDS-PAGE analysis of reduced ab191634 stained overnight with Coomassie Blue. DTT-reduced protein migrates as 45-60 kDa due to glycosylation.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its ability to inhibit anti-CD3 antibody induced IL2 secretion in Human T lymphocytes.
The ED50 for this effect is typically 0.75 - 3 μg/ml.

Accession

Q7Z7D3

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute with sterile deionized water to a concentration of 700 µg/ml.

Storage buffer

pH: 7.4 Constituents: 95% PBS, 5% Trehalose

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"FGISGRHSITVTTVASAGNIGEDGILSCTFEPDIKLSDIVIQWLKEGVLGLVHEFKEGKDELSEQDEMFRGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGNANLEYKTGAFSMPEVNVDYNASSETLRCEAPRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTESEIKRRSHLQLLNSKA","proteinLength":"Fragment","predictedMolecularWeight":"26.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":258,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q7Z7D3","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle|For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B7-H4 also known as VTCN1 or B7x is a member of the B7 family of immunoregulatory proteins. It has an approximate molecular mass of 50 kDa. B7-H4 expression is detected on the surface of various cells including B cells T cells monocytes and some cancer cells. Its expression is often inducible; immune cells can express B7-H4 in response to specific conditions or signals in the environment.
Biological function summary

B7-H4 acts as an immune checkpoint molecule that negatively regulates T cell immunity. It does not form a known complex with other proteins but instead interacts directly with T cells. By inhibiting T cell activation and proliferation B7-H4 contributes to the regulation of immune responses helping to maintain self-tolerance and prevent autoimmunity.

Pathways

B7-H4 is involved in the regulation of immune checkpoints prominently in the pathways related to immune tolerance and suppression. It plays a critical role in the pathways that modulate tumor evasion of the immune system. B7-H4 is related to other immune checkpoint proteins such as PD-L1 which are also pivotal in immune regulation and therapy targets.

B7-H4 is connected to cancer progression and autoimmune conditions. Overexpression of B7-H4 in certain cancers such as ovarian and breast cancer associates with tumor immune evasion allowing tumors to grow unchecked by the immune system. Its expression links with proteins like CTLA-4 in maintaining immune suppression. B7-H4 modifications could serve as a potential target in cancer immunotherapy aiming to enhance the immune system's ability to combat malignancies.
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Specifications

Form

Lyophilized

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General info

Function

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
See full target information VTCN1
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