Recombinant Human B7H4 protein (Biotin) is a Human Fragment protein, in the 29 to 258 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
Amino acid sequence
F G I S G R H S I T V T T V A S A G N I G E D G I L S C T F E P D I K L S D I V I Q W L K E G V L G L V H E F K E G K D E L S E Q D E M F R G R T A V F A D Q V I V G N A S L R L K N V Q L T D A G T Y K C Y I I T S K G K G N A N L E Y K T G A F S M P E V N V D Y N A S S E T L R C E A P R W F P Q P T V V W A S Q V D Q G A N F S E V S N T S F E L N S E N V T M K V V S V L Y N V T I N N T Y S C M I E N D I A K A T G D I K V T E S E I K R R S H L Q L L N S K A H H H H H H H H H H G G G L N D I F E A Q K I E W H E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Alternative names
B7H4, UNQ659/PRO1291, VTCN1, V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4, B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7-H4
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Recombinant Human B7H4 protein (Biotin) is a Human Fragment protein, in the 29 to 258 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
- Accession
- Q7Z7D3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- F G I S G R H S I T V T T V A S A G N I G E D G I L S C T F E P D I K L S D I V I Q W L K E G V L G L V H E F K E G K D E L S E Q D E M F R G R T A V F A D Q V I V G N A S L R L K N V Q L T D A G T Y K C Y I I T S K G K G N A N L E Y K T G A F S M P E V N V D Y N A S S E T L R C E A P R W F P Q P T V V W A S Q V D Q G A N F S E V S N T S F E L N S E N V T M K V V S V L Y N V T I N N T Y S C M I E N D I A K A T G D I K V T E S E I K R R S H L Q L L N S K A H H H H H H H H H H G G G L N D I F E A Q K I E W H E
- Accession
- Q7Z7D3
- Protein length
- Fragment
- Predicted molecular weight
- 28.6 kDa
- Amino acids
- 29 to 258
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
- Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
B7-H4 also known as VTCN1 or B7x is a member of the B7 family of immunoregulatory proteins. It has an approximate molecular mass of 50 kDa. B7-H4 expression is detected on the surface of various cells including B cells T cells monocytes and some cancer cells. Its expression is often inducible; immune cells can express B7-H4 in response to specific conditions or signals in the environment.
- Biological function summary
B7-H4 acts as an immune checkpoint molecule that negatively regulates T cell immunity. It does not form a known complex with other proteins but instead interacts directly with T cells. By inhibiting T cell activation and proliferation B7-H4 contributes to the regulation of immune responses helping to maintain self-tolerance and prevent autoimmunity.
- Pathways
B7-H4 is involved in the regulation of immune checkpoints prominently in the pathways related to immune tolerance and suppression. It plays a critical role in the pathways that modulate tumor evasion of the immune system. B7-H4 is related to other immune checkpoint proteins such as PD-L1 which are also pivotal in immune regulation and therapy targets.
- Associated diseases and disorders
B7-H4 is connected to cancer progression and autoimmune conditions. Overexpression of B7-H4 in certain cancers such as ovarian and breast cancer associates with tumor immune evasion allowing tumors to grow unchecked by the immune system. Its expression links with proteins like CTLA-4 in maintaining immune suppression. B7-H4 modifications could serve as a potential target in cancer immunotherapy aiming to enhance the immune system's ability to combat malignancies.
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1 product image
SDS-PAGE - Recombinant Human B7H4 protein (Biotin) (ab198650)
4-20% SDS Page analysis of ab198650 (2.8 μg)
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