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AB319192

Recombinant Human BAFF/BLyS/TNFSF13B Protein (ECD)

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Recombinant Human BAFF/BLyS/TNFSF13B Protein (ECD) is a Human Fragment protein, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for FuncS, SDS-PAGE.

View Alternative Names

CD257, BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, TNFSF13B, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, B-cell-activating factor, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, BLyS, TALL-1

2 Images
Functional Studies - Recombinant Human BAFF/BLyS/TNFSF13B Protein (ECD) (AB319192)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human BAFF/BLyS/TNFSF13B Protein (ECD) (AB319192)

Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 0.1-1.5 ng/mL.

SDS-PAGE - Recombinant Human BAFF/BLyS/TNFSF13B Protein (ECD) (AB319192)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human BAFF/BLyS/TNFSF13B Protein (ECD) (AB319192)

SDS-PAGE analysis of ab319192

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 0.4-2 ng/mL.

Accession

Q9Y275

Animal free

No

Carrier free

Yes

Species

Human

Storage buffer

Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.1-1.5 ng/mL", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"19 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9Y275","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.
Biological function summary

BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.

Pathways

BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.

BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.
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Specifications

Form

Lyophilized

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General info

Function

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.. Isoform 3. Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.

Sequence similarities

Belongs to the tumor necrosis factor family.

Post-translational modifications

The soluble form derives from the membrane form by proteolytic processing.. Isoform 2 is not efficiently shed from the membrane unlike isoform 1.. N-glycosylated.

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Product protocols

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Target data

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.. Isoform 3. Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
See full target information TNFSF13B
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