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AB93929

Recombinant Human Bag1 protein (Tag Free)

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Recombinant Human Bag1 protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

HAP, BAG1, BAG family molecular chaperone regulator 1, BAG-1, Bcl-2-associated athanogene 1

2 Images
Western blot - Recombinant Human Bag1 protein (Tag Free) (AB93929)
  • WB

Unknown

Western blot - Recombinant Human Bag1 protein (Tag Free) (AB93929)

All lanes:

Anti-Bag1 antibody (<a href='/en-us/products/unavailable/bag1-antibody-ab7976'>ab7976</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Bag1 protein (Tag Free) (ab93929) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human Bag1 protein (AB93929)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Bag1 protein (AB93929)

15% SDS-PAGE showing ab93929 (3 μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Q99933

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p><a href='/en-us/products/unavailable/recombinant-htlv-i-gp46-protein-ab93919'>ab93919</a> can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/bag1-antibody-ab7976'>ab7976</a>.</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q99933","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bag1 protein also known as Bcl-2-associated athanogene 1 has a molecular mass of approximately 46 kDa. The protein is ubiquitously expressed in various tissues and cells indicating its broad functional significance. Bag1 serves as a co-chaperone interacting directly with heat shock protein 70 (Hsp70) to modulate its activity. This interaction affects the protein folding process ensuring cellular proteins maintain their proper conformation.
Biological function summary

Bag1 plays essential roles in regulating cell survival and apoptosis. It can exist as part of a larger protein complex and is known to bind with the anti-apoptotic protein Bcl-2 enhancing cell survival. By interacting with these proteins Bag1 helps control the delicate balance between cell death and survival critical for normal cellular function and response to stress. Bag1 also affects transcription regulation cell cycle progression and cellular response to stress stimuli.

Pathways

Bag1 integrates into cellular stress response and apoptotic signaling pathways. Bag1's interaction with Hsp70 associates it with the heat shock response pathway which upregulates molecular chaperones to protect cells during stress. Additionally its binding to Bcl-2 situates it within the apoptotic pathway where it supports cell survival by inhibiting caspase activation. The protein further interacts with key regulatory proteins like Raf-1 linking it to the MAPK/ERK signaling pathway involved in cell growth and division.

Dysregulation of Bag1 connects to cancer development and neurodegenerative diseases. Overexpression of Bag1 has associations with cancers such as breast cancer where it promotes tumor growth by inhibiting apoptosis. Bag1's ability to interact with the Bcl-2 protein makes it a significant player in cancer pathways. Additionally changes in Bag1 expression and function have been observed in Alzheimer's disease highlighting its role in neuron survival and stress response. Bag1's involvement with proteins like Hsp70 and Bcl-2 highlights its potential as a therapeutic target in these conditions.
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Specifications

Form

Liquid

Additional notes

Purified by using conventional chromatography techniques.

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General info

Function

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed : 24318877, PubMed : 27474739, PubMed : 9873016). Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed : 12724406). Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed : 9305631). Involved in the STUB1-mediated proteasomal degradation of ESR1 in response to age-related circulating estradiol (17-beta-estradiol/E2) decline, thereby promotes neuronal apoptosis in response to ischemic reperfusion injury (By similarity).

Post-translational modifications

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed : 24318877, PubMed : 27474739, PubMed : 9873016). Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed : 12724406). Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed : 9305631). Involved in the STUB1-mediated proteasomal degradation of ESR1 in response to age-related circulating estradiol (17-beta-estradiol/E2) decline, thereby promotes neuronal apoptosis in response to ischemic reperfusion injury (By similarity).
See full target information BAG1
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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB32109

Anti-Bag1 antibody [Y166]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Bag1 antibody [Y166] (AB32109)

  • 5
  • 4
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99695

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain

  • 0
  • 0

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