Recombinant Human Bak protein is a Human Full Length protein, in the 1 to 211 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A S G Q G P G P P R Q E C G E P A L P S A S E E Q V A Q D T E E V F R S Y V F Y R H Q Q E Q E A E G V A A P A D P E M V T L P L Q P S S T M G Q V G R Q L A I I G D D I N R R Y D S E F Q T M L Q H L Q P T A E N A Y E Y F T K I A T S L F E S G I N W G R V V A L L G F G Y R L A L H V Y Q H G L T G F L G Q V T R F V V D F M L H H C I A R W I A Q R G G W V A A L N L G N G P I L N V L V V L G V V L L G Q F V V R R F F K S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
Alternative names
BAK, BCL2L7, CDN1, BAK1, Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7
Recommended products
Recombinant Human Bak protein is a Human Full Length protein, in the 1 to 211 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q16611-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M A S G Q G P G P P R Q E C G E P A L P S A S E E Q V A Q D T E E V F R S Y V F Y R H Q Q E Q E A E G V A A P A D P E M V T L P L Q P S S T M G Q V G R Q L A I I G D D I N R R Y D S E F Q T M L Q H L Q P T A E N A Y E Y F T K I A T S L F E S G I N W G R V V A L L G F G Y R L A L H V Y Q H G L T G F L G Q V T R F V V D F M L H H C I A R W I A Q R G G W V A A L N L G N G P I L N V L V V L G V V L L G Q F V V R R F F K S
- Accession
- Q16611
- Protein length
- Full Length
- Predicted molecular weight
- 49.28 kDa
- Amino acids
- 1 to 211
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
- Sequence similarities
Belongs to the Bcl-2 family.
- Subcellular localisation
- Mitochondrion outer membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Bak protein also known as Bcl-2 homologous antagonist killer plays a mechanical role in the mitochondrial apoptosis pathway. It often functions as a pro-apoptotic member of the Bcl-2 protein family. Bak is expressed widely in the body being present in tissues such as the brain and heart. The molecular weight of Bak is approximately 23 kilodaltons. Bak interacts with other mitochondrial proteins to trigger cell death processes.
- Biological function summary
Bak engages in promoting apoptosis by disrupting the mitochondrial outer membrane potential. It is part of the Bcl-2 family complex which balances cell survival and cell death. Bak collaborates with Bax protein to form pores in the mitochondrial membrane releasing cytochrome c and other apoptotic factors. This process initiates the cascade of caspases leading to programmed cell death.
- Pathways
Bak is a critical component of the intrinsic apoptotic pathway. It directly interacts with pro-apoptotic proteins like Bax and anti-apoptotic proteins such as Bcl-2 and Bcl-xL. Bak's action in the apoptotic pathway involves a delicate balance between survival and death signals affecting cellular homeostasis. In addition Bak's interaction with the p53 pathway emphasizes its role in response to DNA damage ensuring damaged cells do not proliferate uncontrollably.
- Associated diseases and disorders
Malfunction or dysregulation of Bak can lead to cancer and neurodegenerative disorders. In cancer reduced Bak activity may allow cells to evade apoptosis promoting tumor survival and growth. Conversely in diseases like Parkinson's excessive Bak activity may result in enhanced neuronal apoptosis. Bak's interaction with proteins such as Bcl-2 also makes it a potential target for therapies aimed at restoring apoptotic balance in affected cells.
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1 product image
SDS-PAGE - Recombinant Human Bak protein (ab114337)
ab114337 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue
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