Recombinant Human BAP1 protein is a Human Full Length protein, in the 1 to 729 aa range, expressed in Wheat germ, with >=80% purity and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Purity
- >=80%
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M N K G W L E L E S D P G L F T L L V E D F G V K G V Q V E E I Y D L Q S K C Q G P V Y G F I F L F K W I E E R R S R R K V S T L V D D T S V I D D D I V N N M F F A H Q L I P N S C A T H A L L S V L L N C S S V D L G P T L S R M K D F T K G F S P E S K G Y A I G N A P E L A K A H N S H A R P E P R H L P E K Q N G L S A V R T M E A F H F V S Y V P I T G R L F E L D G L K V Y P I D H G P W G E D E E W T D K A R R V I M E R I G L A T A G E P Y H D I R F N L M A V V P D R R I K Y E A R L H V L K V N R Q T V L E A L Q Q L I R V T Q P E L I Q T H K S Q E S Q L P E E S K S A S N K S P L V L E A N R A P A A S E G N H T D G A E E A A G S C A Q A P S H S P P N K P K L V V K P P G S S L N G V H P N P T P I V Q R L P A F L D N H N Y A K S P M Q E E E D L A A G V G R S R V P V R P P Q Q Y S D D E D D Y E D D E E D D V Q N T N S A L R Y K G K G T G K P G A L S G S A D G Q L S V L Q P N T I N V L A E K L K E S Q K D L S I P L S I K T S S G A G S P A V A V P T H S Q P S P T P S N E S T D T A S E I G S A F N S P L R S P I R S A N P T R P S S P V T S H I S K V L F G E D D S L L R V D C I R Y N R A V R D L G P V I S T G L L H L A E D G V L S P L A L T E G G K G S S P S I R P I Q G S Q G S S S P V E K E V V E A T D S R E K T G M V R P G E P L S G E K Y S P K E L L A L L K C V E A E I A N Y E A C L K E E V E K R K K F K I D D Q R R T H N Y D E F I C T F I S M L A Q E G M L A N L V E Q N I S V R R R Q G V S I G R L H K Q R K P D R R K R S R P Y K A K R Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed:12485996, PubMed:18757409, PubMed:20436459, PubMed:25451922, PubMed:35051358). Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed:20436459, PubMed:25451922, PubMed:30664650, PubMed:35051358). Does not deubiquitinate monoubiquitinated histone H2B (PubMed:20436459, PubMed:30664650). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed:20805357, PubMed:30664650, PubMed:36180891). Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination (PubMed:30664650). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed:36180891). Recruited to specific gene-regulatory regions by YY1 (PubMed:20805357). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed:19188440, PubMed:19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed:19188440, PubMed:19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed:19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed:19117993). Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed:34170818).
Alternative names
KIAA0272, hucep-6, BAP1, Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1, Cerebral protein 6
Recommended products
Recombinant Human BAP1 protein is a Human Full Length protein, in the 1 to 729 aa range, expressed in Wheat germ, with >=80% purity and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
- >=80%
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q92560-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M N K G W L E L E S D P G L F T L L V E D F G V K G V Q V E E I Y D L Q S K C Q G P V Y G F I F L F K W I E E R R S R R K V S T L V D D T S V I D D D I V N N M F F A H Q L I P N S C A T H A L L S V L L N C S S V D L G P T L S R M K D F T K G F S P E S K G Y A I G N A P E L A K A H N S H A R P E P R H L P E K Q N G L S A V R T M E A F H F V S Y V P I T G R L F E L D G L K V Y P I D H G P W G E D E E W T D K A R R V I M E R I G L A T A G E P Y H D I R F N L M A V V P D R R I K Y E A R L H V L K V N R Q T V L E A L Q Q L I R V T Q P E L I Q T H K S Q E S Q L P E E S K S A S N K S P L V L E A N R A P A A S E G N H T D G A E E A A G S C A Q A P S H S P P N K P K L V V K P P G S S L N G V H P N P T P I V Q R L P A F L D N H N Y A K S P M Q E E E D L A A G V G R S R V P V R P P Q Q Y S D D E D D Y E D D E E D D V Q N T N S A L R Y K G K G T G K P G A L S G S A D G Q L S V L Q P N T I N V L A E K L K E S Q K D L S I P L S I K T S S G A G S P A V A V P T H S Q P S P T P S N E S T D T A S E I G S A F N S P L R S P I R S A N P T R P S S P V T S H I S K V L F G E D D S L L R V D C I R Y N R A V R D L G P V I S T G L L H L A E D G V L S P L A L T E G G K G S S P S I R P I Q G S Q G S S S P V E K E V V E A T D S R E K T G M V R P G E P L S G E K Y S P K E L L A L L K C V E A E I A N Y E A C L K E E V E K R K K F K I D D Q R R T H N Y D E F I C T F I S M L A Q E G M L A N L V E Q N I S V R R R Q G V S I G R L H K Q R K P D R R K R S R P Y K A K R Q
- Accession
- Q92560
- Protein length
- Full Length
- Predicted molecular weight
- 106.8 kDa
- Amino acids
- 1 to 729
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Glutathione Sepharose
General info
- Function
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed:12485996, PubMed:18757409, PubMed:20436459, PubMed:25451922, PubMed:35051358). Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed:20436459, PubMed:25451922, PubMed:30664650, PubMed:35051358). Does not deubiquitinate monoubiquitinated histone H2B (PubMed:20436459, PubMed:30664650). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed:20805357, PubMed:30664650, PubMed:36180891). Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination (PubMed:30664650). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed:36180891). Recruited to specific gene-regulatory regions by YY1 (PubMed:20805357). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed:19188440, PubMed:19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed:19188440, PubMed:19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed:19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed:19117993). Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed:34170818).
- Sequence similarities
Belongs to the peptidase C12 family. BAP1 subfamily.
- Post-translational modifications
Ubiquitinated: monoubiquitinated at multiple sites within its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention (PubMed:24703950). Able to mediate autodeubiquitination via intramolecular interactions to counteract cytoplasmic retention (PubMed:24703950). Monoubiquitinated on at least 4 sites near or within its PY-NLS (PubMed:35446349).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human BAP1 protein (ab152849)
12.5% SDS-PAGE analysis of ab152849 stained with Coomassie Blue.
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