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AB268359

Recombinant human BAP1 protein (Active)

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(1 Publication)

Recombinant human BAP1 protein (Active) is a Human Full Length protein, in the 1 to 729 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA0272, hucep-6, BAP1, Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1, Cerebral protein 6

2 Images
Functional Studies - Recombinant human BAP1 protein (Active) (AB268359)
  • FuncS

Unknown

Functional Studies - Recombinant human BAP1 protein (Active) (AB268359)

The specific activity of ab268359 was ≥245 nmol/min/mg in a deubiquitinase assay using recombinant hman ubiquitin-based proluciferin as substrate.

SDS-PAGE - Recombinant human BAP1 protein (Active) (AB268359)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human BAP1 protein (Active) (AB268359)

SDS-PAGE analysis of ab268359.

Key facts

Purity

>70% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268359 was ≥245 nmol/min/mg in a deubiquitinase assay using recombinant hman ubiquitin-based proluciferin as substrate.

Accession

Q92560

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB255611

Anti-BAP1 antibody [EPR22826-65]

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Proteins & Peptides

AB160346

Recombinant Human alpha Internexin protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human alpha Internexin protein (GST tag N-Terminus) (AB160346)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGAEEAAGSCAQAPSHSPPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTNSALRYKGKGTGKPGALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPLSIKTSSGAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGVLSPLALTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPGEPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":729,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q92560","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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General info

Function

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed : 12485996, PubMed : 18757409, PubMed : 20436459, PubMed : 25451922, PubMed : 35051358). Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed : 20436459, PubMed : 25451922, PubMed : 30664650, PubMed : 35051358). Does not deubiquitinate monoubiquitinated histone H2B (PubMed : 20436459, PubMed : 30664650). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed : 20805357, PubMed : 30664650, PubMed : 36180891). Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination (PubMed : 30664650). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed : 36180891). Recruited to specific gene-regulatory regions by YY1 (PubMed : 20805357). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed : 19188440, PubMed : 19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed : 19188440, PubMed : 19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed : 19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed : 19117993). Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed : 24703950). Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed : 34170818).

Sequence similarities

Belongs to the peptidase C12 family. BAP1 subfamily.

Post-translational modifications

Ubiquitinated: monoubiquitinated at multiple sites within its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention (PubMed:24703950). Able to mediate autodeubiquitination via intramolecular interactions to counteract cytoplasmic retention (PubMed:24703950). Monoubiquitinated on at least 4 sites near or within its PY-NLS (PubMed:35446349).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed : 12485996, PubMed : 18757409, PubMed : 20436459, PubMed : 25451922, PubMed : 35051358). Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed : 20436459, PubMed : 25451922, PubMed : 30664650, PubMed : 35051358). Does not deubiquitinate monoubiquitinated histone H2B (PubMed : 20436459, PubMed : 30664650). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed : 20805357, PubMed : 30664650, PubMed : 36180891). Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination (PubMed : 30664650). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed : 36180891). Recruited to specific gene-regulatory regions by YY1 (PubMed : 20805357). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed : 19188440, PubMed : 19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed : 19188440, PubMed : 19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed : 19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed : 19117993). Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed : 24703950). Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed : 34170818).
See full target information BAP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 13:3382-3399 PubMed37655321

2023

Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1-BARD1, suppresses DNA repair and sensitizes CRC to radiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yue,Tingyu Liu,Xuecen Wang,Weijian Wu,Gesi Wen,Yang Yi,Jiaxin Wu,Ziyang Wang,Weixiang Zhan,Ruirui Wu,Yuan Meng,Zhirui Cao,Liyuan Le,Wenyan Qiu,Xiaoyue Zhang,Zhenyu Li,Yong Chen,Guohui Wan,Xianzhang Bu,Zhenwei Peng,Ran-Yi Liu
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