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AB196402

Recombinant human BAZ2B protein (His tag N-Terminus)

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Recombinant human BAZ2B protein (His tag N-Terminus) is a Human Fragment protein, in the 2054 to 2168 aa range, expressed in Escherichia coli, with >84%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA1476, BAZ2B, Bromodomain adjacent to zinc finger domain protein 2B, hWALp4

2 Images
Functional Studies - Recombinant human BAZ2B protein (His tag N-Terminus) (AB196402)
  • FuncS

Supplier Data

Functional Studies - Recombinant human BAZ2B protein (His tag N-Terminus) (AB196402)

Example of specific activity of ab196402.

SDS-PAGE - Recombinant human BAZ2B protein (His tag N-Terminus) (AB196402)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human BAZ2B protein (His tag N-Terminus) (AB196402)

4-20% SDS-PAGE analysis of ab196402 (5 μg) with Coomassie staining.

Key facts

Purity

>84% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Accession

Q9UIF8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB234420

Anti-BAZ2B antibody [EPR20909-88]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-BAZ2B antibody [EPR20909-88] (AB234420)

  • 0
  • 0
Biochemicals

AB141525

3-Morpholinosydnonimine hydrochloride (SIN-1 hydrochloride), Vasodilator

Chemical Structure - 3-Morpholinosydnonimine hydrochloride (SIN-1 hydrochloride), Vasodilator (AB141525)

  • 0
  • 0
Reagents, Controls & Accessories

AB104135

Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield (AB104135)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MHHHHHHSVKKPKRDDSKDLALCSMILTEMETHEDAWPFLLPVNLKLVPGYKKVIKKPMDFSTIREKLSSGQYPNLETFALDVRLVFDNCETFNEDDSDIGRAGHNMRKYFEKKWTDTFKVS","proteinLength":"Fragment","predictedMolecularWeight":"14.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":2168,"aminoAcidStart":2054,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UIF8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

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General info

Function

Regulatory subunit of the ATP-dependent BRF-1 and BRF-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed : 28801535). Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). The BRF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the BRF-5 ISWI chromatin remodeling complex (PubMed : 28801535). Chromatin reader protein, which may play a role in transcriptional regulation via interaction with ISWI (By similarity) (PubMed : 10662543). Involved in positively modulating the rate of age-related behavioral deterioration (By similarity). Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with histone methyltransferase EHMT1 (By similarity).

Sequence similarities

Belongs to the WAL family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Regulatory subunit of the ATP-dependent BRF-1 and BRF-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed : 28801535). Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). The BRF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the BRF-5 ISWI chromatin remodeling complex (PubMed : 28801535). Chromatin reader protein, which may play a role in transcriptional regulation via interaction with ISWI (By similarity) (PubMed : 10662543). Involved in positively modulating the rate of age-related behavioral deterioration (By similarity). Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with histone methyltransferase EHMT1 (By similarity).
See full target information BAZ2B
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Product promise

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