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AB132142

Recombinant Human BCAR1 protein

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Recombinant Human BCAR1 protein is a Human Full Length protein, in the 1 to 870 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CAS, CASS1, CRKAS, BCAR1, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, Cas scaffolding protein family member 1, p130cas

1 Images
SDS-PAGE - Recombinant Human BCAR1 protein (AB132142)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human BCAR1 protein (AB132142)

12.5% SDS-PAGE analysis of ab132142 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

P56945

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVLGQLAAA","proteinLength":"Full Length","predictedMolecularWeight":"119.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":870,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P56945","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (PubMed : 12432078, PubMed : 12832404). Implicated in induction of cell migration and cell branching (PubMed : 12432078, PubMed : 12832404, PubMed : 17038317). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).

Sequence similarities

Belongs to the CAS family.

Post-translational modifications

PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.. Dephosphorylated by PTPN14 at Tyr-128.. Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner; phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway.

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Product protocols

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Target data

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (PubMed : 12432078, PubMed : 12832404). Implicated in induction of cell migration and cell branching (PubMed : 12432078, PubMed : 12832404, PubMed : 17038317). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).
See full target information BCAR1
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Complementary Products

Primary Antibodies

AB108320

Anti-BCAR1 antibody [EPR1850(2)]

RabMAb|Recombinant

Western blot - Anti-BCAR1 antibody [EPR1850(2)] (AB108320)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB70204

Anti-MYO1D antibody

Western blot - Anti-MYO1D antibody (AB70204)

  • 0
  • 0
Primary Antibodies

AB190217

Anti-B3GNT7 antibody

Western blot - Anti-B3GNT7 antibody (AB190217)

  • 0
  • 0

Product promise

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