Recombinant Human Bcr protein is a Human Fragment protein, in the 1 to 695 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >75% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M V D P V G F A E A W K A Q F P D S E P P R M E L R S V G D I E Q E L E R C K A S I R R L E Q E V N Q E R F R M I Y L Q T L L A K E K K S Y D R Q R W G F R R A A Q A P D G A S E P R A S A S R P Q P A P A D G A D P P P A E E P E A R P D G E G S P G K A R P G T A R R P G A A A S G E R D D R G P P A S V A A L R S N F E R I R K G H G Q P G A D A E K P F Y V N V E F H H E R G L V K V N D K E V S D R I S S L G S Q A M Q M E R K K S Q H G A G S S V G D A S R P P Y R G R S S E S S C G V D G D Y E D A E L N P R F L K D N L I D A N G G S R P P W P P L E Y Q P Y Q S I Y V G G M M E G E G K G P L L R S Q S T S E Q E K R L T W P R R S Y S P R S F E D C G G G Y T P D C S S N E N L T S S E E D F S S G Q S S R V S P S P T T Y R M F R D K S R S P S Q N S Q Q S F D S S S P P T P Q C H K R H R H C P V V V S E A T I V G V R K T G Q I W P N D G E G A F H G D A D G S F G T P P G Y G C A A D R A E E Q R R H Q D G L P Y I D D S P S S S P H L S S K G R G S R D A L V S G A L E S T K A S E L D L E K G L E M R K W V L S G I L A S E E T Y L S H L E A L L L P M K P L K A A A T T S Q P V L T S Q Q I E T I F F K V P E L Y E I H K E F Y D G L F P R V Q Q W S H Q Q R V G D L F Q K L A S Q L G V Y R A F V D N Y G V A M E M A E K C C Q A N A Q F A E I S E N L R A R S N K D A K D P T T K N S L E T L L Y K P V D R V T R S T L V L H D L L K H T P A S H P D H P L L Q D A L R I S Q N F L S S I N E E I T P R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed:17116687, PubMed:1903516, PubMed:7479768). The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed:23940119, PubMed:7479768). The amino terminus contains an intrinsic kinase activity (PubMed:1657398). Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed:17116687). Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed:23940119).
Alternative names
BCR1, D22S11, BCR, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26
Recommended products
Recombinant Human Bcr protein is a Human Fragment protein, in the 1 to 695 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >75% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P11274-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.29% Sodium chloride, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- M V D P V G F A E A W K A Q F P D S E P P R M E L R S V G D I E Q E L E R C K A S I R R L E Q E V N Q E R F R M I Y L Q T L L A K E K K S Y D R Q R W G F R R A A Q A P D G A S E P R A S A S R P Q P A P A D G A D P P P A E E P E A R P D G E G S P G K A R P G T A R R P G A A A S G E R D D R G P P A S V A A L R S N F E R I R K G H G Q P G A D A E K P F Y V N V E F H H E R G L V K V N D K E V S D R I S S L G S Q A M Q M E R K K S Q H G A G S S V G D A S R P P Y R G R S S E S S C G V D G D Y E D A E L N P R F L K D N L I D A N G G S R P P W P P L E Y Q P Y Q S I Y V G G M M E G E G K G P L L R S Q S T S E Q E K R L T W P R R S Y S P R S F E D C G G G Y T P D C S S N E N L T S S E E D F S S G Q S S R V S P S P T T Y R M F R D K S R S P S Q N S Q Q S F D S S S P P T P Q C H K R H R H C P V V V S E A T I V G V R K T G Q I W P N D G E G A F H G D A D G S F G T P P G Y G C A A D R A E E Q R R H Q D G L P Y I D D S P S S S P H L S S K G R G S R D A L V S G A L E S T K A S E L D L E K G L E M R K W V L S G I L A S E E T Y L S H L E A L L L P M K P L K A A A T T S Q P V L T S Q Q I E T I F F K V P E L Y E I H K E F Y D G L F P R V Q Q W S H Q Q R V G D L F Q K L A S Q L G V Y R A F V D N Y G V A M E M A E K C C Q A N A Q F A E I S E N L R A R S N K D A K D P T T K N S L E T L L Y K P V D R V T R S T L V L H D L L K H T P A S H P D H P L L Q D A L R I S Q N F L S S I N E E I T P R
- Accession
- P11274
- Protein length
- Fragment
- Predicted molecular weight
- 120 kDa
- Amino acids
- 1 to 695
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed:17116687, PubMed:1903516, PubMed:7479768). The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed:23940119, PubMed:7479768). The amino terminus contains an intrinsic kinase activity (PubMed:1657398). Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed:17116687). Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed:23940119).
- Post-translational modifications
Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Bcr protein also known as breakpoint cluster region protein plays an important role in cellular signaling. It has a molecular mass of approximately 160 kDa and is expressed in many cell types such as hematopoietic cells. Bcr functions as a GTPase-activating protein (GAP) for p21rac and other small GTP-binding proteins. This activity influences cellular processes such as growth and differentiation. Bcr is also known for its involvement in the formation of the Bcr-Abl fusion protein an important factor in certain leukemias.
- Biological function summary
The Bcr protein acts within cells to regulate signal transduction and cytoskeletal organization. It is part of the pre-B cell receptor (pre-BCR) signaling complex influencing B-cell development. Its GAP activity is important for the regulation of small GTPases impacting cell migration and adhesion. Alterations in Bcr function can affect cellular responses such as apoptosis making it a focus for research into targeted therapies for hematological conditions.
- Pathways
The Bcr protein is an important player in signaling pathways involving the Ras superfamily of small GTPases. It is notably involved in the Rho GTPase pathway tied to cytoskeletal dynamics and cell cycle progression. Bcr's interaction with these pathways puts it in relation to proteins like Rac and Cdc42 which are vital for various cellular processes. Disruption in Bcr-associated pathways can lead to aberrant cell growth and metastasis.
- Associated diseases and disorders
Bcr has a well-documented link to certain leukemias particularly chronic myeloid leukemia (CML). The Bcr-Abl fusion protein resulting from the translocation of chromosomes 9 and 22 is central to CML pathology. Additionally Bcr connects with other proteins such as Abl in this disease context. Further disruptions in Bcr signaling can relate to disorders involving immune system dysregulation demonstrating the protein's relevance in both oncology and immunology research.
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1 product image
SDS-PAGE - Recombinant Human Bcr protein (ab72493)
SDS-PAGE showing ab72493 at approximately 120kDa.
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