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Recombinant human BD-3 protein is a Human Full Length protein, in the 23 to 67 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

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Key facts

Purity
>98% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
Escherichia coli
Tags
Tag free
Applications
SDS-PAGE, FuncS, HPLC
Biologically active
Yes

Amino acid sequence

G I I N T L Q K Y Y C R V R G G R C A V L S C L P K E E Q I G K C S T R G R K C C R R K K

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.

Alternative names

BD3, DEFB103, DEFB3, DEFB103B, DEFB103A, Beta-defensin 103, Beta-defensin 3, Defensin-like protein, BD-3, DEFB-3, HBD3, hBD-3

Recommended products

Recombinant human BD-3 protein is a Human Full Length protein, in the 23 to 67 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

Key facts

Purity
>98% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
Escherichia coli
Applications
SDS-PAGE, FuncS, HPLC
Biological activity
Exhibits anti-microbial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E.coli.
Accession
P81534-1
Animal free
No
Species
Human
Reconstitution
Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C or -80°C. Further dilutions should be made in appropriate buffered solutions.
Concentration
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Storage buffer

pH: 7.4
Constituents: 99% Phosphate Buffer, 0.75% Sodium chloride

Sequence info

Amino acid sequence

G I I N T L Q K Y Y C R V R G G R C A V L S C L P K E E Q I G K C S T R G R K C C R R K K
Accession
P81534
Protein length
Full Length
Predicted molecular weight
5.1 kDa
Amino acids
23 to 67
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

Purity >98% by SDS-PAGE and HPLC analyses.

General info

Function

Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.

Sequence similarities

Belongs to the beta-defensin family.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle, Reconstitute for long term storage

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Previously labelled as beta Defensin 3.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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    Product protocols

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