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AB200245

Recombinant human BD-3 protein

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(1 Publication)

Recombinant human BD-3 protein is a Human Full Length protein, in the 23 to 67 aa range, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

BD3, DEFB103, DEFB3, DEFB103B, DEFB103A, Beta-defensin 103, Beta-defensin 3, Defensin-like protein, BD-3, DEFB-3, HBD3, hBD-3

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE, HPLC

applications

Biologically active

Yes

Biological activity

Exhibits anti-microbial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E.coli.

Accession

P81534

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C or -80°C. Further dilutions should be made in appropriate buffered solutions.

Storage buffer

pH: 7.4 Constituents: 99% Phosphate Buffer, 0.75% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as beta Defensin 3.
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Sequence info

[{"sequence":"GIINTLQKYYCRVRGGRCAVLSCLPKEEQIGKCSTRGRKCCRRKK","proteinLength":"Full Length","predictedMolecularWeight":"5.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":67,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P81534","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Reconstitute for long term storage
True
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Specifications

Form

Lyophilized

Additional notes

Purity >98% by SDS-PAGE and HPLC analyses.

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General info

Function

Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.

Sequence similarities

Belongs to the beta-defensin family.

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Product protocols

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Target data

Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.
See full target information DEFB103A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta virologica 62:287-293 PubMed30160144

2018

Induction and antiviral activity of human β-defensin 3 in intestinal cells with picornavirus infection.

Applications

Unspecified application

Species

Unspecified reactive species

W Chen,Z Liu,Q Zhang,Q Yan,S Jing
View all publications
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