Recombinant Human Beclin 1 protein is a Human Full Length protein, in the 1 to 450 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M E G S K T S N N S T M Q V S F V C Q R C S Q P L K L D T S F K I L D R V T I Q E L T A P L L T T A Q A K P G E T Q E E E T N S G E E P F I E T P R Q D G V S R R F I P P A R M M S T E S A N S F T L I G E A S D G G T M E N L S R R L K V T G D L F D I M S G Q T D V D H P L C E E C T D T L L D Q L D T Q L N V T E N E C Q N Y K R C L E I L E Q M N E D D S E Q L Q M E L K E L A L E E E R L I Q E L E D V E K N R K I V A E N L E K V Q A E A E R L D Q E E A Q Y Q R E Y S E F K R Q Q L E L D D E L K S V E N Q M R Y A Q T Q L D K L K K T N V F N A T F H I W H S G Q F G T I N N F R L G R L P S V P V E W N E I N A A W G Q T V L L L H A L A N K M G L K F Q R Y R L V P Y G N H S Y L E S L T D K S K E L P L Y C S G G L R F F W D N K F D H A M V A F L D C V Q Q F K E E V E K G E T R F C L P Y R M D V E K G K I E D T G G S G G S Y S I K T Q F N S E E Q W T K A L K F M L T N L K W G L A W V S S Q F Y N K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.
Alternative names
GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197
Recommended products
Recombinant Human Beclin 1 protein is a Human Full Length protein, in the 1 to 450 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q14457-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E G S K T S N N S T M Q V S F V C Q R C S Q P L K L D T S F K I L D R V T I Q E L T A P L L T T A Q A K P G E T Q E E E T N S G E E P F I E T P R Q D G V S R R F I P P A R M M S T E S A N S F T L I G E A S D G G T M E N L S R R L K V T G D L F D I M S G Q T D V D H P L C E E C T D T L L D Q L D T Q L N V T E N E C Q N Y K R C L E I L E Q M N E D D S E Q L Q M E L K E L A L E E E R L I Q E L E D V E K N R K I V A E N L E K V Q A E A E R L D Q E E A Q Y Q R E Y S E F K R Q Q L E L D D E L K S V E N Q M R Y A Q T Q L D K L K K T N V F N A T F H I W H S G Q F G T I N N F R L G R L P S V P V E W N E I N A A W G Q T V L L L H A L A N K M G L K F Q R Y R L V P Y G N H S Y L E S L T D K S K E L P L Y C S G G L R F F W D N K F D H A M V A F L D C V Q Q F K E E V E K G E T R F C L P Y R M D V E K G K I E D T G G S G G S Y S I K T Q F N S E E Q W T K A L K F M L T N L K W G L A W V S S Q F Y N K
- Accession
- Q14457
- Protein length
- Full Length
- Predicted molecular weight
- 78.3 kDa
- Amino acids
- 1 to 450
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense.
- Sequence similarities
Belongs to the beclin family.
- Post-translational modifications
Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy (PubMed:19180116). In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy (PubMed:23878393, PubMed:25891078).
- Subcellular localisation
- Endosome membrane, Mitochondrion membrane, Endosome, Mitochondrion, Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.
- Biological function summary
The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.
- Pathways
Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.
- Associated diseases and disorders
Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.
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1 product image
SDS-PAGE - Recombinant Human Beclin 1 protein (ab131770)
12.5% SDS-PAGE analysis of ab131770 stained with Coomassie Blue.
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