Recombinant Human Bestrophin/BEST1 protein is a Human Full Length protein, in the 1 to 604 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M F E K L T L Y C D S Y I Q L I P I S F V L G F Y V T L V V T R W W N Q Y E N L P W P D R L M S L V S G F V E G K D E Q G R L L R R T L I R Y A N L G N V L I L R S V S T A V Y K R F P S A Q H L V Q A G F M T P A E H K Q L E K L S L P H N M F W V P W V W F A N L S M K A W L G G R I R D P I L L Q S L L N E M N T L R T Q C G H L Y A Y D W I S I P L V Y T Q V V T V A V Y S F F L T C L V G R Q F L N P A K A Y P G H E L D L V V P V F T F L Q F F F Y V G W L K V A E Q L I N P F G E D D D D F E T N W I V D R N L Q V S L L A V D E M H Q D L P R M E P D M Y W N K P E P Q P P Y T A A S A Q F R R V S F M G S T F N I S L N K E E M E F Q P N Q E D E E D A H A G I I G R F L G L Q S H D H H P P R A N S R T K L L W P K R E S L L H E G L P K N H K A A K Q N V R G Q E D N K A W K L K A V D A F K S A P L Y Q R P G Y Y S A P Q T P L S P T P M F F P L E P S A P S K L H S V T G I D T K D K S L K T V S S G A K K S F E L L S E S D G A L M E H P E V S Q V R R K T V E F N L T D M P E I P E N H L K E P L E Q S P T N I H T T L K D H M D P Y W A L E N R S V L H L N Q G H C I A L C P T P A S L A L S L P F L H N F L G F H H C Q S T L D L R P A L A W G I Y L A T F T G I L G K C S G P F L T S P W Y H P E D F L G P G E G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed:11904445, PubMed:12907679, PubMed:18179881, PubMed:18400985, PubMed:19853238, PubMed:21330666, PubMed:26200502, PubMed:26720466, PubMed:35789156). Allows the movement of chloride and hydrogencarbonate (PubMed:11904445, PubMed:12907679, PubMed:18179881, PubMed:18400985, PubMed:19853238, PubMed:21330666, PubMed:26200502, PubMed:26720466, PubMed:35789156). Found in a partially open conformation leading to significantly smaller chloride movement (PubMed:35789156). Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition (By similarity). Releases the 4-aminobutanoate neurotransmitter in a (Ca2+)-dependent manner, and participates in its tonic release from cerebellar glial cells (By similarity).
Alternative names
VMD2, BEST1, Bestrophin-1, TU15B, Vitelliform macular dystrophy protein 2
Recommended products
Recombinant Human Bestrophin/BEST1 protein is a Human Full Length protein, in the 1 to 604 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- O76090-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M F E K L T L Y C D S Y I Q L I P I S F V L G F Y V T L V V T R W W N Q Y E N L P W P D R L M S L V S G F V E G K D E Q G R L L R R T L I R Y A N L G N V L I L R S V S T A V Y K R F P S A Q H L V Q A G F M T P A E H K Q L E K L S L P H N M F W V P W V W F A N L S M K A W L G G R I R D P I L L Q S L L N E M N T L R T Q C G H L Y A Y D W I S I P L V Y T Q V V T V A V Y S F F L T C L V G R Q F L N P A K A Y P G H E L D L V V P V F T F L Q F F F Y V G W L K V A E Q L I N P F G E D D D D F E T N W I V D R N L Q V S L L A V D E M H Q D L P R M E P D M Y W N K P E P Q P P Y T A A S A Q F R R V S F M G S T F N I S L N K E E M E F Q P N Q E D E E D A H A G I I G R F L G L Q S H D H H P P R A N S R T K L L W P K R E S L L H E G L P K N H K A A K Q N V R G Q E D N K A W K L K A V D A F K S A P L Y Q R P G Y Y S A P Q T P L S P T P M F F P L E P S A P S K L H S V T G I D T K D K S L K T V S S G A K K S F E L L S E S D G A L M E H P E V S Q V R R K T V E F N L T D M P E I P E N H L K E P L E Q S P T N I H T T L K D H M D P Y W A L E N R S V L H L N Q G H C I A L C P T P A S L A L S L P F L H N F L G F H H C Q S T L D L R P A L A W G I Y L A T F T G I L G K C S G P F L T S P W Y H P E D F L G P G E G R
- Accession
- O76090
- Protein length
- Full Length
- Predicted molecular weight
- 95.5 kDa
- Amino acids
- 1 to 604
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed:11904445, PubMed:12907679, PubMed:18179881, PubMed:18400985, PubMed:19853238, PubMed:21330666, PubMed:26200502, PubMed:26720466, PubMed:35789156). Allows the movement of chloride and hydrogencarbonate (PubMed:11904445, PubMed:12907679, PubMed:18179881, PubMed:18400985, PubMed:19853238, PubMed:21330666, PubMed:26200502, PubMed:26720466, PubMed:35789156). Found in a partially open conformation leading to significantly smaller chloride movement (PubMed:35789156). Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition (By similarity). Releases the 4-aminobutanoate neurotransmitter in a (Ca2+)-dependent manner, and participates in its tonic release from cerebellar glial cells (By similarity).
- Sequence similarities
Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Bestrophin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bestrophin also known as BEST1 or VMD2 is a protein that functions as a calcium-activated chloride channel. It plays an important role in the transport of ions across cell membranes. The molecular mass of BEST1 is about 68 kDa. This protein is prominently expressed in the retinal pigment epithelium (RPE) which is a layer of cells in the eye vital to the function and survival of the photoreceptors. These photoreceptors are responsible for capturing light and initiating vision.
- Biological function summary
BEST1 influences the regulation of ionic homeostasis in the eye and contributes to the RPE's maintenance. It acts as part of a chloride channel complex that helps transport chloride ions which is important for fluid absorption and secretion in the retina. There are interactions with calcium ions that activate the channel allowing it to regulate the flow of other ions in response to changes in calcium concentration which is essential for maintaining the ionic balance necessary for proper vision.
- Pathways
BEST1 plays significant roles in the visual cycle and ion transport pathways in the retina. It is related to the protein ANO2 another chloride channel although they interact differently with calcium ions. BEST1 influences the fluid regulation within the retina an essential part of the visual cycle pathway. It signals the movement of ions in response to external stimuli maintaining the balance of fluids and ions that reh cells function correctly.
- Associated diseases and disorders
Mutations in the BEST1 gene are connected to Best vitelliform macular dystrophy and adult-onset foveomacular dystrophy. Both disorders lead to retinal degeneration affecting vision. The interaction between BEST1 and other chloride channels like ANO2 suggests possible effects on the severity and progression of these retinal diseases highlighting the need for continued research into therapeutic interventions and the complex network of protein interactions within the retina.
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1 product image
SDS-PAGE - Recombinant Human Bestrophin/BEST1 protein (ab152797)
12.5% SDS-PAGE showing ab152797 stained with Coomassie Blue.
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